Browsing by Author "Sabuncuoglu, Bizden"

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    The Effect of Temperature and Contact Time of Sodium Hypochlorite on Human Roots Infected with Enterococcus Faecalis and Candida Albicans
    (2014) Gulsahi, Kamran; Tirali, R. Ebru; Cehreli, S. Burcak; Karahan, Zeynep Ceren; Uzunoglu, Emel; Sabuncuoglu, Bizden; https://orcid.org/0000-0003-3510-7265; https://orcid.org/0000-0001-6487-3984; 23011474; AAX-5565-2021; AAD-2907-2020; AAD-6138-2021
    The aim of this study was to evaluate the effectiveness of 2.5 % NaOCl at different temperature and time intervals on Enterococcus faecalis and Candida albicans-infected human roots. A total of 112 root cylinders prepared from extracted single-rooted humans were infected by E. faecalis (Group A, n = 56) or C. albicans (Group B, n = 56); 3 root cylinders served as negative controls. Both groups were further divided into 6 subgroups according to three contact times (30 s, 1 min, 5 min) with NaOCl at two different temperatures (25 or 37 A degrees C). Microorganism growth was controlled at the 24th and 48th hours. Statistical analysis was performed using the Chi-square test. While NaOCl at 25 A degrees C for 5 min was the most effective irrigation regimen to eliminate E. faecalis (p < 0.001), NaOCl at 37 A degrees C for 5 min exhibited significantly superior antifungal properties (p < 0.05). At the same contact times, difference in the temperature of NaOCl did not affect the growth of either E. faecalis or C. albicans. As a result, the irrigation time of NaOCl was more effective than the temperature to eliminate E. faecalis, while pre-heating of NaOCl to 37 A degrees C increased its effectiveness on C. albicans at 5 min contact time.