Browsing by Author "Orun, Huseyin"

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Comparison of Three Different Rotavirus Antigen Tests for Rotavirus Detection in Fecal Samples: A Retrospective Analysis
(2022) Kirdar, Sevin; Erol, Nural; Kahyaoglu, Fadime; Yazici, Vesile; Orun, Huseyin; Altindis, Mustafa; https://orcid.org/0000-0002-4384-0881; AAG-9736-2019
Objective: Direct antigen tests are the most commonly used methods in most laboratories to detect rotavirus rapidly in stool samples. This study aimed to evaluate the performance of three commercially available test methods for detecting rotaviruses in fecal specimens and compare the results with those of the reverse transcription-polymerase chain reaction (RT-PCR), which is considered a gold standard test.Materials and Methods: The presence of rotavirus antigens in stool samples was investigated by an enzyme-linked immunosorbent assay (ELISA), an immunochromatographic test (ICT), and a latex agglutination test (LAT), which were commercially available. The results of these tests were compared with those of a multiplex RT-PCR as a reference test. Sensitivity, specificity, and positive and negative predictive values were calculated, and agreement with RT-PCR was evaluated by Cohen's kappa test.Results: A total of 85 patients (51.8% male and 48.2% female, aged 0-32 years) were included in this study. The sensitivities of the ICT, LAT, and ELISA tests were 78.6%, 78.6%, and 96.4%, respectively; the specificities of the tests were 69.0%, 72.4%, and 69.0%, respectively. According to kappa tests, moderate agreement was found between RT-PCR and ICT (Kappa=0.464, p<0.001); moderate agreement was found between RT-PCR and LAT (Kappa=0.493, p<0.001); substantial agreement was found between RT-PCR and ELISA (Kappa=0.694, p<0.001). The ELISA test showed the highest sensitivity and a high level of agreement with RT-PCR.Conclusion: ICT and LAT are quick and practical tests for rotavirus detection. However, in this study, it was seen that they were not superior to the ELISA test in terms of accuracy of diagnosis.
Expanded New Born Bloodspot Screening: Developed Country Examples And Shat Can Be Done In Turkey
(2022) Fidan, Caglar; Orun, Huseyin; Alper, Asli Begum; Unver, Cigdem Naz; Sahin, Omer Can; Ugurlu, Zeynep; Akdur, Recep; Taruscio, Domenica; https://orcid.org/0000-0002-4384-0881; https://orcid.org/0000-0002-1713-4342; 35702584; AAG-9736-2019; GPW-7632-2022
Bloodspot screening in newborns is an exemplary public health intervention as it is essential secondary prevention with proven efficacy and benefit for the early diagnosis and prompt treatment of rare diseases. In this mini review, newborn bloodspot screening (NBS) programs of 12 countries were examined in terms of the extent of diseases/disorders screened to form recommendations for Turkey's expanded newborn screening program. Essentially, Turkey and 11 selected countries' official policies/ national programs or strategies in terms of newborn screening and the number of diseases/conditions screened were examined. The current status of spinal muscular atrophy (SMA) screening was also checked through the SMA NBS Alliance. In addition, WHO and EURORDIS guidelines for newborn screening were also reviewed. On the Pubmed database, following the search strategy "((newborn screening[Title/Abstract]) OR (newborn screening program[Title/Abstract])) OR (newborn blood spot screening[Title/Abstract])" in the PubMed database from 1 January 2008 to 1 December 2021. Diseases that will be recommended to be included in the Turkish national newborn bloodspot screening program will be presented by evaluating the updated criteria of Wilson and Jungner by constructing international comparisons. The number of diseases/disordeis screened by the inspected 12 countries is eminently variable and ranges from 5 in Turkey to 51 in New York, United States of America (USA). Acknowledging the programs of other countries, it is evident that Turkey must advance its program by evaluating the epidemiological data in Turkey, the health workforce, and infrastructure while relying on the updated screening criteria. The newborn bloodspot screening program should be expanded based on the cost estimates and implemented starting with pilot applications and the diseases/disorders that are deemed appropriate should be included in the national program.
The Prevalence of University Students? Use of Tobacco Products, Their Opinions on Their Effects on Health, and the Factors Affecting Use
(2022) Aykut, Nihal Bilgili; Orun, Huseyin; Topal, Ecenur; https://orcid.org/0000-0002-4384-0881
Objective: It was aimed to examine the prevalence of use of tobacco products, the reasons for the first use, opinions on tobacco products' effects on health, and factors affecting the waterpipe use of university students enrolled in technical, social, and health sciences in this study.Materials and Methods: This cross-sectional study was conducted with a 43 -item questionnaire among fourth-year students from three educational fields of a university with 72.3% participation rate was in Ankara, Turkey in March 2019.Results: 21.1% of the students were current waterpipe users and 32.6% were current cigarette smokers. For both cigarette and waterpipe, "curiosity" was the most common reason first reported use, followed by socializing and related reasons. For both tobacco products, there was consensus on whether they were harmful to health and on the positive effects of quitting. Regarding the addictive effect, significantly more of the participants gave lower scores for waterpipe than for cigarettes (46.4% versus 83.5%). The risk factors for being current waterpipe use were the students' cigarette smoking and waterpipe use in the family or close friends.Conclusion: Waterpipe use was relatively common among university students and socialization was important as a risk factor for being a current user. The addictive effect of waterpipe can be emphasized more in awareness campaigns. The fight against tobacco should be conducted by peer work and targeting all tobacco products and regardless of students' educational fields.