Browsing by Author "Mutlu, Ahmet"

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Akustik travmaya bağlı oluşan işitme kaybında sentetik adrenokortikotropik hormon tedavisinin koruyuculuk etkisinin araştırılması
(Başkent Üniversitesi Sağlık Bilimleri Enstitüsü, 2017) Mutlu, Ahmet; Özlüoğlu, Levent N.
Adrenokortikotropik hormon (ACTH) hipofiz glandın ön lobunda üretilen polipeptid yapıda bir hormondur. En önemli etkisi, adrenal korteksten kortikosteroid hormonların (glukokortikoidkortizon ve mineralokortikoid- aldosteron) salgılanmasını uyarmasıdır. Bu çalışmada sentetik ACTH analoglarının (Synacthen®) akustik travmaya maruz bırakılmış ratlardaki koruyuculuk etkisinin deksametazonun koruyuculuk etkisi ile karşılaştırılması amaçlanmıştır. Materyal ve Metod Bu çalışma Başkent Üniversitesi Tıp Fakültesi Deney Hayvanları Araştırma ve Uygulama Merkezinde (DA 16/47 proje nolu) etik kurul onayı alınarak yapılmıştır. 6 sıçandan oluşan 4 farklı grubun 10 kHz, 20 kHz, 32 kHz frekanslarında olmak üzere ABR ölçümleri yapılarak bazal işitme eşikleri belirlendikten sonra gruplardan 3 tanesine 105 dB beyaz gürültü ile 12 saatlik akustik travma verilmiştir. Bütün hayvanların ölçümleri akustik travma sonrası tekrarlandıktan sonra 7 gün süreyle akustik travma alan bir gruba (n:6) deksametazon, bir gruba (n:6) sentetik ACTH analoğu, bir gruba (n:6) serum fizyolojik uygulanmıştır. Uygulama sonrası 7. ve 21 gün ABR ölçümleri tekrarlanarak işitme eşikleri belirlenmiştir. Elde edilen veriler verilerin dağılımına uygun olarak istatistiksel yöntemler ile değerlendirilmiştir. Bulgular Kontrol grubu haricindeki bütün hayvanlar akustik travmadan benzer şekilde etkilendiği bulunmuştur. Steroid ve ACTH gruplarında serum fizyolojik verilen gruba göre 7. ve 21. günlerde yapılan ölçümlerinde bütün frekanslarda anlamlı olarak iyileşme izlenmiştir. Steroid ve ACTH grupları arasında yapılan karşılaştırmada anlamlı farklılık izlenmemiştir. Kontrol grubu haricindeki grupların işitme eşikleri bazal ölçümler ile aynı olmadıkları izlenmiştir. Sonuç Akustik travmaya karşı sistemik ACTH analoglarının uygulamasının etkin olduğu izlenmiş olup, literatürde önerilen sistemik steroid etkinliğine benzer etki gösterdiği belirlenmiştir. ACTH analogları, insanlar ile yapılacak ileri araştırmalar sonucunda steroidlere alternatif bir tedavi yöntemi olabilir. Introduction Adrenocorticotropic hormon (ACTH) is polypeptid hormon which is secreted from anterior pituitary gland. The main aim of this hormone is to stimulate the secretation of corticosteroid hormones from the adrenal glands. In this study, we aimed to compare the protective effects of the ACTH and dexamethasone against the acoustic trauma. Material and Methods This study is claimed in the Baskent University Medical Faculty animal lab under the local ethic statement (DA16/47). Hearing thresholds and wave latancies are detected for 3 different frequencies (10 kHz, 20 kHz, 32 kHz) with auditory brainstem response test (ABR) in 4 groups which constituted with randomized selected 6 rats. 3 groups received acoustic trauma (105 dB white noise, 12 hours) and all of the rats are assessed immediately for the threshold shifting. One group (n:6) received dexamethasone (2 mg/kg/day im), one group (n:6) received ACTH (0,4 mg/kg/day im), one group (n:6) received saline (0,2 ml/day im) for seven days. ABR test were revised in 7. and 21. of acoustic trauma. Results All of the groups (except the control group) were similarly effected from the acoustic trauma. Steroid and ACTH groups show significantly better hearing outcomes than the saline group on 7th and 21st days after the acoustic. The results between the steroid and ACTH analogs did not differ significantly. None of the related intervention groups reached the basal hearing thresholds. Conclusion It is found in the study, both systemic steroids and ACTH analogs exert a therapeutic effect on acoustic trauma. ACTH analogs may an alternative treatment for the systemic steroids after the further investments on human.
Audiological and vestibular measurements in Behcet's disease
(2019) Ertugrul, Ozlem; Mutlu, Ahmet; Zindanci, Ilkin; Cam, Osman Halit; Ozluoglu, Levent; 30929056; AAP-1533-2020
IntroductionBehcet's disease (BD) is a vasculitis that involves all small vessels and influences the multiple systems of the human body. This study aimed to evaluate the audio-vestibular system involvement of patients with BD and healthy individuals.Materials and methodsThis study was designed as a prospective case-control blinded study. Thirty-one patients with BD and 31 healthy individuals were included. All the subjects were evaluated via pure tone audiometry (PTA), video head impulse test (vHIT), post head shake nystagmus test (PHSNT) and dizziness handicap inventory (DHI) to check for audio-vestibular system involvement.ResultsPatients with BD showed higher PTA scores in both speech and high frequencies. The vHIT revealed pathological saccades, particularly in horizontal canals (right ear: p=0.002, left ear: p=0.039). The gain values of the patients were slightly lower than those of the control group; however, gain and gain asymmetry differed significantly in a few canals. In the spontaneous nystagmus test and PHSNT, pathological nystagmus was detected to be significantly higher in the patient group than control group (p=0.001); but the saccade presence in vHIT and nystagmus in PHNT did not differ among the patients (p=0.106). In addition, the DHI scores of the patients group were higher than those of the control group (p<0.001). No correlation was found between disease duration and saccade presence.ConclusionThe vHIT was used preliminary for evaluating the vestibular system in BD. This study showed the influence of BD on the audio-vestibular system, in particular isolated horizontal canal involvement was discovered in patients with BD.Level of evidenceLevel III b.
The Protective Effect of Adrenocorticotropic Hormone Treatment Against Noise-Induced Hearing Loss
(2018) Mutlu, Ahmet; Ocal, Fatma Ceyda Akin; Erbek, Seyra; Ozluoglu, Levent; 0000-0001-9022-921X; 0000-0002-8453-6069; 0000-0002-2150-0237; 29747961; AAI-2097-2019; AAJ-2445-2021; AAI-8020-2021
Objective: NIHL is a common problem, and steroids are the most effective treatment option. In this study, we aimed to evaluate the protective effects of the synthetic adrenocorticotropic hormone (ACTH) analogues, which induce endogenous steroid secretion, against noise-induced hearing loss (NIHL) and to compare their effectiveness with that of steroid treatment. Methods: Twenty-four male Sprague Dawley albino rats were divided into four subgroups as follows: group 1 (n = 6) control, group 2 (n = 6) saline, group 3 (n = 6) dexamethasone (2 mg/kg/ day intramuscularly [IM]), group 4 (n = 6) ACTH analogue (0,4 mg/kg/day IM), respectively. Three groups (groups 2-4) were exposed to white noise (105 dB SPL, 12 h). All the rats were evaluated for hearing thresholds of 10 kHz, 20 kHz, and 32 kHz via acoustic brainstem responses (ABR) measurement. After the basal threshold measurements, measurements were repeated immediately after the noise and on day 7 and day 21. Results: Both steroid and ACTH analogue groups showed significantly better hearing outcomes than the saline group on day 7 (p < 0.001) and day 21 (p < 0.001) after the noise exposure. No superior treatment effect was demonstrated in either the steroid or ACTH analogue group. None of the related intervention groups reached the basal hearing thresholds. Conclusion: Steroids were effective drugs for the treatment of NIHL. ACTH analogues also demonstrated promising therapeutic effects for NIHL. Further studies to establish ACTH analogues as an alternative NIHL treatment option to steroids are needed. (C) 2018 Elsevier B.V. All rights reserved.