Browsing by Author "Kulaksizoglu, Sevsen"

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Aqueous Humour and Serum Levels of Nitric Oxide, Malondialdehyde and Total Antioxidant Status in Patients with Type 2 Diabetes with Proliferative Diabetic Retinopathy and Nondiabetic Senile Cataracts
(2016) Kulaksizoglu, Sevsen; https://orcid.org/0000-0002-7613-2240; 26388409; AAI-8932-2021
Objectives: Diabetic retinopathy is associated with enhanced oxidative stress and/or reduction of antioxidant status. Several studies have examined the oxidative agents, such as nitric oxide (NO) and malondialdehyde (MDA), in the body fluids of patients with diabetes. However, total antioxidant status (TAS) in the aqueous humour of patients with diabetes has not been determined to date. To address this issue, we analyzed the aqueous humour and serum levels of NO, MDA and TAS in patients with type 2 diabetes and nondiabetic senile cataracts. Methods: This prospective study included 35 patients with type 2 diabetes and 35 age- and sex-matched healthy subjects in whom cataract surgery was indicated. Aqueous humour and serum MDA, NO and TAS levels were determined by spectrophotometric methods, respectively. Results: The analysis of MDA levels in the serum and aqueous humour revealed no significant differences in any of the groups (p>0.05). At the level of aqueous humour, patients with type 2 diabetes had significantly increased NO levels, compared to the controls (p=0.003). The control group also presented significantly higher TAS levels than the subgroup with type 2 diabetes in serum (p=0.001). However, there were no significant differences in the TAS levels of aqueous humour and serum NO levels in the groups (p>0.05). Conclusions: Our results seem to demonstrate that the development of diabetic retinopathy is associated with high levels of aqueous humour NO and reduced serum antioxidant defenses. Therefore, inhibition of reactive oxygen species production and substitution of serum antioxidant status may be a therapeutic target for eye diseases associated with oxidative stress. (C) 2016 Canadian Diabetes Association. Published by Elsevier Inc. All rights reserved.
The effect of pregabalin on tourniquet-induced ischemia-reperfusion injury: a prospective randomized study
(2019) Karaca, Omer; Pinar, Huseyin Ulas; Ozgur, Ahmet Fevzi; Kulaksizoglu, Sevsen; Dogan, Rafi; 0000-0003-0473-6763; 31655508; Q-2420-2015
Background/aim: The aim of this study was to investigate the efficacy of pregabalin on ischemia-reperfusion injuries. Materials and methods: Fifty-four patients were randomly assigned into 2 groups. A 150-mg tablet of pregabalin was given the night before and then 1 h before the operation for patients in Group P (pregabalin group, n = 27). A placebo was given to patients in Group C (control group, n = 27) at the same times. After combined spinal-epidural anesthesia was performed, venous blood samples were taken before tourniquet inflation (t(1)), just before tourniquet deflation (t(2)), and 20 min after tourniquet deflation (t(3)) for the analysis of total antioxidant status (TAS), total oxidant status (TOS), catalase (CAT), and ischemia-modified albumin (IMA). Results: There was no significant difference in TAS levels between the groups for the t(3) period. However, the TAS in Group P was significantly higher in the t(3) period than the t(2) period (mean +/- SD, 0.46 +/- 0.1 vs. 0.38 +/- 0.2 mmol of Trolox equivalent/L, respectively; P < 0.05). The CAT level in the C period was significantly higher in Group P than Group C (mean +/- SD, 53.04 +/- 32.1 vs. 35.46 +/- 17.2 mu mol/formaldehyde, respectively; P < 0.05). In the t(3) period, the TOS was significantly lower in Group P than Group C (mean +/- SD, 11.97 +/- 5 vs. 18.29 +/- 9.9 pg/mL, respectively; P < 0.05). The TOS in Group P was significantly lower in the t(3) period than the t(2) period (mean +/- SD, 11.97 +/- 5 vs. 18.98 +/- 10.7 pg/mL, respectively; P < 0.0001). Conclusion: Pregabalin has no marked antioxidant activity, but it contributes to the antioxidant defense system of an organism.
Importance of laboratory parameters in patients with obstructive sleep apnea and their relationship with cardiovascular diseases
(2018) Kulaksizoglu, Sevsen; Kivanc, Tulay; Lakadamyali, Huseyin; Eyuboglu, Fusun; 0000-0002-7613-2240; 0000-0002-5525-8207; 28345811; AAI-8932-2021; AAR-4338-2020
BackgroundOstructive sleep apnea (OSA) is an independent risk factor for the development of cardiovascular events. Platelet activation and inflammation are the mechanisms involved in the association between OSA and cardiovascular disease (CVD). The markers of platelet activation and inflammation are the mean platelet volume (MPV), platelet-lymphocyte ratio (PLR), red cell distribution width (RDW), neutrophil- lymphocyte ratio (NLR). We aimed to define the association of NLR, PLR, RDW, and MPV with the severity of disease and the presence of CVD. MethodsThis study consisted of 300 patients who were admitted to the sleep laboratory. The patients were classified according to their apnea- hypopnea index (AHI) scores as OSA negative (Group A: AHI<5), mild (Group B: AHI: 5-15), moderate (Group C: AHI=15-30), and severe OSA (Group D: AHI >30). ResultsThere were no significant differences in the NLR, PLR, and MPV among the groups (P>.05); only RDW differed significantly (P=.04). RDW was significantly higher in patients with than without risk factors for CVD [15.6% (15.4-15.7) vs 15.3% (15.1-15.3), respectively; P=.02]. ConclusionsNLR, PLR, MPV, and RDW are widely available and easily obtained from a routinely performed hemogram. Among these laboratory parameters, only RDW can demonstrate the reverse consequences of OSA-associated comorbidities, because vascular damage due to systemic inflammation is an important underlying mechanism in these diseases. RDW might be used as a marker of the response and patient compliance with continuous positive airway pressure treatment.
IS THERE A REAL CORRELATION BETWEEN RED CELL DISTRIBUTION WIDTH AND PERIPHERAL ARTERIAL DISEASE?
(2017) Duman, Enes; Kulaksizoglu, Sevsen; Cifci, Egemen; Ozulku, Mehmet; 0000-0002-7613-2240; 30581327; AAI-8932-2021
Background: Few data is available concerning the association between peripheral arterial disease (PAD) and red cell distribution width (RDW). In this study, we analyzed the relationship between RDW and atherosclerosis of the vessels other than coronary arteries in patients who had undergone digital substraction angiography (DSA). Methods: This study included 730 patients who had undergone DSA. Patients were divided into two groups according to their angiographic images. The association between RDW and atherosclerosis of peripheral arteries was analyzed. The relationship between atherosclerosis and smoking, hypertension (HT), diabetes mellitus (DM), hs-CRP, hemoglobin, white blood cell (WBC), triglyceride, total cholesterol, HDL and LDL cholesterol levels was assessed. Results: Atherosclerosis was observed more common in male and patients with older age, HT, DM and smoking (p< 0.001). hs-CRP and WBC levels were both in significantly positive association with atherosclerosis (p< 0.05). However, there were no significant differences in the RDW levels, hemoglobin, triglyceride, total cholesterol, LDL and HDL cholesterol levels in the groups (p> 0.05). Conclusions: Our results seem to demonstrate that older age, male gender, HT, DM and smoking are powerful risk factors for PAD. In contrast to the previous reports, RDW levels are found not to be associated with atherosclerosis of peripheral arteries.
Oxidative Stress and Nasal Polyposis: Does It Affect The Severity of The Disease?
(2014) Topal, Ozgul; Kulaksizoglu, Sevsen; Erbek, Selim S.; https://orcid.org/0000-0001-6305-5023; https://orcid.org/0000-0002-7613-2240; https://orcid.org/0000-0003-4825-3499; 24717866; ABI-6777-2020; AAI-8932-2021; B-7604-2019
Background: Nasal polyposis (NP) is a chronic inflammatory disease and the waste products of this inflammation are reactive oxygen species composed of free radicals. Changes in oxidative status have already been revealed in NP. The aim of this study was to investigate the effect of oxidative status to the severity of the disease and the quality of life. Methods: The study group included 24 patients with NP and 20 controls. The Turkish version of the Rhinosinusitis Disability Index, visual analog scale (VAS), polyp stage, computed tomography (CT) score, and the eosinophilic cationic protein (ECP) levels in nasal lavage (NAL) fluid were used to assess the severity of the disease. Malondialdehyde, nitric oxide (NO), and the total antioxidant status (TAS) levels in NAL fluids were measured representing the oxidative stress. Results: NO values were correlated with nasal congestion (p = 0.031). TAS values were correlated with nasal obstruction (p = 0.039). ECP values showed correlation with all the nasal obstruction (p = 0.003), congestion (p = 0.009), rhinorrhea (p = 0.009), and VAS scores (p = 0.039). Conclusion: In NP, ECP levels detected in NAL fluid were significantly high and were correlated with the severity of the disease. Moreover, the severity of oxidative stress, in the forms of TAS and NO, is significantly correlated with the severity of the nasal obstruction and congestion, respectively.
Parathyroid Hormone Levels in the Prediction of Ischemic Stroke Risk
(2017) Dener, Sefik; Celik, Guner; Dogan, Ali; Ozturk, Serefnur; Kulaksizoglu, Sevsen; Ekmekci, Hakan; 0000-0002-7613-2240; 28115793; AAI-8932-2021
Objective. It was examined whether PTH and 25-dihydroxyvitamin D(25(OH)D) levels, together or separately, are indicators of the risk of stroke. Materials and Methods. This prospective study was performed at two centers. In the study, 100 patients diagnosed with acute ischemic stroke and 100 control individuals in the same age range were examined. In addition to neurological examination, cranial imaging, extensive routine blood chemistry, PTH, and 25(OH) D levels were evaluated in all cases. Stroke risk factors were determined. Logistic regression was used for statistical analysis. Results. A total of 60 patients and 79 control individuals were included in the study. Different estimation models were designed in order to examine the relationship between PTH and 25(OH) D levels with stroke. According to modeling results, it was determined that the most effective predictor for risk of stroke was 25(OH) D levels, followed by hypertension and PTH levels, respectively. Conclusion. PTH and 25(OH) D levels together can make important contributions to determination of stroke risk, and further investigations are needed to understand this relationship more fully.
A Prospective Clinical Study of Flow-Mediated Dilatation in Burn Injury
(2014) Turk, Emin; Caliskan, Mustafa; Karagulle, Erdal; Aydogan, Cem; Oguz, Hakan; Kulaksizoglu, Sevsen; Yildirim, Erkan; Moray, Gokhan; Haberal, Mehmet; https://orcid.org/0000-0003-4766-3373; https://orcid.org/0000-0002-8522-4956; https://orcid.org/0000-0003-1547-1297; https://orcid.org/0000-0002-7613-2240; https://orcid.org/0000-0002-9057-722X; https://orcid.org/0000-0003-2498-7287; https://orcid.org/0000-0002-3462-7632; 24165669; AAJ-5609-2021; JYO-9455-2024; C-6247-2017; AAJ-5296-2021; AAI-8932-2021; ABI-3856-2020; AAE-1041-2021; AAJ-8097-2021
The Relationship Between Vitamin B12 and Vitamin D Levels and Subjective Cognitive Complaints in Patients with First Episode Major Depressive Disorder
(2021) Ulusoy, Selen Isik; Horasanli, Bahriye; Kulaksizoglu, Sevsen; 0000-0003-3142-1011
Objective: Cognitive dysfunction is an important component of major depressive disorder (MDD). The aim of this study is to investigate the relationship between vitamin B12 and D and subjective cognitive complaints in patients with first-episode MDD. Method: In this cross-sectional study, patients (n=120) diagnosed with first-episode MDD filled out self-report questionnaires that evaluate subjective neurocognitive symptoms (Perceived Deficits Questionnaire-Depression [PDQ-D] and British Columbia Cognitive Complaints Inventory [BC-CCI]). Disease severity was evaluated using the Hamilton Depression Rating Scale (HDRS). Serum 25-OH vitamin D and vitamin B12 levels were measured using a chemiluminescence microparticle immunoassay technology. Results: The PDQ-D and BC-CCI scores were significantly higher in patients with combined vitamin B12 and D deficiency than patients with normal vitamin levels. We found a negative correlation between vitamin B12 level and HDRS, BC-CCI and PDQ-D scores. In addition, there was a positive correlation between HDRS scores and PDQ-D and BC-CCI scores. Linear regression analysis revealed that HDRS score and vitamin B12 level have influence on PDQ-D and BC-CCI scores. Conclusion: In this study, it was found that subjective cognitive impairment was significantly higher in MDD patients with both of vitamin B12 and vitamin D deficiency. Vitamin B12 levels were also associated with depressive symptom severity and subjective cognitive impairment. Depression severity and vitamin B12 level were found to be significant predictors of subjective cognitive impairment. Our findings highlight the importance of depression severity and vitamin B12 and D levels during routine clinical evaluation of MDD.
Role of mean platelet volume in pregnancy to predict gestational diabetes mellitus in the first trimester
(2020) Colak, Eser; Ozcimen, Emel Ebru; Ceran, Mehmet Ufuk; Tohma, Yusuf Aytac; Kulaksizoglu, Sevsen; 0000-0001-9418-4733; 0000-0002-7613-2240; 0000-0003-1923-2373; 0000-0002-8184-7531; 30947572; AAE-6482-2021; AAI-8932-2021
Objectives:Gestational diabetes mellitus (GDM) is defined as glucose intolerance detected for the first time in pregnancy or identified during pregnancy. Mean platelet volume (MPV) is a marker of activation and function of platelet. Several studies investigated the relation between MPV and GDM. The purpose of the present study is to predict GDM in the first trimester by using MPV value. Materials and methods:Two hundred pregnant women with GDM and 200 normal pregnant women were enrolled in the study. The first trimester MPV values of GDM and control groups were compared to predict GDM in the first trimester. Results:Women with GDM had higher MPV value compared with the control group (p < .001). The area under the receiver-operator curve was 0.70 for MPV. The cutoff value of MPV was 7.38 fl with 70% sensitivity and 60% specificity. According to the ages, MPV value was higher in GDM group in the individuals who were above 28-year-old (p < .001). Conclusion:MPV can be used to predict GDM in the first trimester.
Role of prolidase activity and oxidative stress biomarkers in unexplained infertility
(2021) Isbilen, Elif; Kulaksizoglu, Sevsen; Kirmizioglu, Mahmut; Komurcu, Ozge Karuserci; Tabur, Suzan; 34449881
Objective Our aim was to explore the significance of serum prolidase enzyme activity and oxidative stress in women with unexplained infertility (UEI). Methods In this case-control study (n = 160; 86 cases; 74 controls) prolidase enzyme activity and total antioxidant status (TAS), total oxidant status (TOS), oxidative stress index (OSI), and vitamin E were measured in plasma using enzyme-linked immunosorbent assays. Results Prolidase enzyme activity and TAS levels were particularly higher in the patient group (P = 0.013, P = 0.001, respectively). Decreased OSI levels were detected in the patient group (P = 0.001). There was a positive relationship of prolidase with vitamin E in both patient and control groups (r = 0.892, P = 0.001, and r = 0.659, P = 0.001, respectively). A positive, but weak, relationship was identified between prolidase activity and TOS levels and also between vitamin E and TOS levels in the UEI group (r = 0.265, P = 0.049, and r = 0.288, P = 0.014, respectively). No association was found between prolidase and TOS levels or between vitamin E and TOS levels in the control group (r = 0.0097, P = 0.527, and r = 0.085, P = 0.610, respectively). Conclusion Our results showed an association between serum prolidase activity and oxidative stress in UEI patients. Further studies including greater groups are required to show the role of reactive oxygen species in UEI.
The Role of the Central and the Peripheral Neuropeptides in Weight Gain and Metabolic Changes Related to Olanzapine
(2021) Arpaci, Nazile; Ak, Mehmet; Uguz, Faruk; Kurban, Sevil; Kulaksizoglu, Sevsen
Objective: This study aimed to examine the role of central and peripheral neuropeptides in olanzapine-induced weight gain and metabolic changes. Materials and Methods: Thirty patients who would receive olanzapine treatment were evaluated at the beginning of the treatment at the 2nd and 8th weeks. Weight, waist circumference, the central neuropeptides pro-opiomelanocortin (POMC) and neuropeptide Y (NPY), and the peripheral adipokine leptin and the peripheral peptide cholecystokinin (CCK) levels were measured in each control. In addition, biochemical parameters such as fasting blood glucose (FBG), aspartate aminotransferase (AST), alanine aminotransferase (ALT), gamma-glutamyl transferase (GGT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), total and direct bilirubin, very-low-density lipoprotein (VLDL), low-density lipoprotein (LDL), high-density lipoprotein (HDL), total cholesterol, and triglyceride (TG) were measured. Results: There were statistically significant differences weight and waist circumference levels compared to the initial levels. As observed in previous studies in the literature, changes in biochemical parameters including AST, ALT, total and direct bilirubin, LDL, TG, total cholesterol, and HDL levels were statistically significant. Levels of the neuropeptides POMC and NPY tended to increase at early stages and decrease at later stages of the treatment, while CCK and leptin levels kept increasing throughout the treatment period. The changes in POMC and CCK levels were statistically significant. Conclusion: The results suggest that POMC and CCK may play a role in olanzapine-related weight gain.
Serum and placental periostin levels in women with early pregnancy loss
(2020) Eroglu, Semra; Colak, Eser; Erinanc, Ozgur Hilal; Ozdemir, Duygu; Ceran, Mehmet Ufuk; Tasdemir, Umit; Kulaksizoglu, Sevsen; Ozcimen, Emel Ebru; 0000-0003-1401-6356; 0000-0002-7613-2240; 0000-0002-9959-9433; 32460058; AAL-1268-2021; AAI-8932-2021
Objectives: Periostin is secreted from the placenta in the embryonic period and it is emphasized that it may be involved in endometrial implantation. In this study, we aimed to investigate periostin serum levels and placental tissue expression in first trimester pregnancy losses. Study design: In this prospective case-control study, 30 patients who underwent dilatation and curettage with first trimester spontaneous abortion (< 10 weeks of gestation) were included in the study group and 30 patients who had voluntary pregnancy termination (< 10 gestational weeks) were included in the control group. Serum samples collected from the study and control groups were analyzed usingenzyme-linkedimmunosorbent assay (ELISA), and trophoblastic and decidual tissues were examined using immunohistochemical staining with streptavidin-biotin-peroxidase techniques. Results: There were no significant differences between the groups in terms of age, gravida status, parity number, gestational week, and number of previous abortions. In the spontaneous abortion group, the serum level of periostin was significantly lower than in the voluntary termination group (6.56 +/- 4.16 pg/mLvs. 9.51 +/- 4.52 pg/mL, p = 0.03). There was no significant difference between the two groups in terms of periostin expression in decidual and trophoblastic tissue (p = 0.617, p = 0.274, p = 0.497). Conclusion: Periostin serum levels were significantly reduced in patients with spontaneous pregnancy loss. Periostin can be used as a predictive marker for the success of endometrial implantation.
Vitamin D receptor gene TaqI single nucleotide polymorphism is not associated with lead levels in maternal and umbilical cord blood
(2019) Tohma, Yusuf Aytac; Akad, Selin; Colak, Eser; Kulaksizoglu, Sevsen; Akyol, Mesut; Terzi, Yunus Kasim; Ozcimen, Emel Ebru; Esin, Sertac; Sahin, Feride Iffet; 0000-0001-5612-9696; 0000-0001-7308-9673; 0000-0002-3808-7004; 0000-0001-9418-4733; 0000-0002-8184-7531; 29463156; AAC-8356-2020; B-4372-2018; AAC-7232-2020
Purpose: We aimed to investigate the association of vitamin D receptor (VDR) gene TaqI single nucleotide polymorphism (SNPs) with serum lead (Pb) levels in maternal and umbilical cord blood. Materials and methods: Eighty-one patients who lived in Konya, Turkey for the last 3 years and had delivery at Baskent University Konya Hospital in 2016 were included in this study. Venous blood samples were drawn from each volunteer immediately before giving birth to determine the maternal Pb levels and VDR SNPs. Additionally, umbilical cord blood samples were collected from the umbilical vein into tube with EDTA as an anticoagulant immediately after birth to determine Pb levels of the fetus. Results: The median level of Pb in the maternal blood was 29.00 (Interquartile Range (IQR) = 16.35) mu g/L and the median Pb level in the cord blood was 22.50 (IQR = 9.75) mu g/L. Blood Pb level of women living in the urban area was significantly higher than in those living in the rural area (Z = 2.118; p = .034). There was a very strong positive correlation between the Pb levels in the maternal blood and in the umbilical cord blood (rho = 0.825, p < .001, respectively). Regarding VDR SNPs, "TT", "TC", and "CC" VDR TaqI genotypes were observed in 28 (34.6%), 45 (55.5%), and eight samples (9.9%), respectively. Pb levels in maternal and cord blood were higher in women with the "CC" VDR TaqI genotype; however, there was no statistically significant difference (p > .05). Conclusions: Although women with the "CC" VDR TaqI genotype had higher maternal and cord blood Pb levels, this was statistically insignificant and therefore, VDR TaqI SNPs did not significantly affect maternal and umbilical cord blood Pb levels.