Browsing by Author "Korpe, Didem Aksoy"

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Alleviation of Copper Induced Oxidative Damage and Genotoxicity in Tomato by Corchourus Olitorius and Urtica Pilulifera Extracts
(2014) Iseri, Ozlem Darcansoy; Korpe, Didem Aksoy; Sahin, Feride Iffet; Haberal, Mehmet; https://orcid.org/0000-0001-7308-9673; https://orcid.org/0000-0002-3462-7632; AAC-7232-2020; AAJ-8097-2021
Biosynthesis of Metallic Nanoparticles: A Comparative Optimization Study for Plant Leaf Extracts and Their Biological Assays
(2016) Korpe, Didem Aksoy; Iseri, Ozlem Darcansoy; Duman, Memed; Denkbas, Emir Baki; Sahin, Feride Iffet; Haberal, Mehmet; 0000-0002-2616-6733; 0000-0001-7308-9673; 0000-0002-3462-7632; B-9957-2014; AAC-7232-2020; AAJ-8097-2021
Corchorus olitorius L. (Jute) leaf and seed extracts exerted high antibacterial activity against food and plant pathogenic bacteria
(2022) Iseri, Ozlem Darcansoy; Korpe, Didem Aksoy; Sahin, Feride Iffet; Cabi, Evren; Haberal, Mehmet; 0000-0002-3462-7632; AAJ-8097-2021
Aim of this study was to comparatively evaluate antibacterial activities of methanol (MetOH), acetone (Ace), petroleum ether (PE) and aqueous (dw) leaf (L), root (R), and seed (S) extracts of Corchorus olitorius L. on both food- and plant-borne pathogens, with DPPH radical scavenging activities (DRSA), and quantitative and qualitative constituent analysis. Leaf PE has the highest strain susceptibility on both food- and plant-borne pathogens. Clavibacter michiganensis, Pseudomonas tomato, and Erwinia caratovora were susceptible to nearly all the leaf and seed extracts. Very low minimum inhibitory concentration (8-128 mL(-1)) and minimum bactericidal concentration (32-2048 mu g mL(-1)) were determined for both leaf and seed extracts against C. michiganensis. Total phenolic contents were correlated to DRSA. The phenolic compounds tested were higher in the leaf MetOH, cholorogenic acid being the most abundant one. Palmitic acid was determined in leaf PE and seed PE extracts. Results presented here demonstrate high antibacterial activity of C. olitorius leaf seed extracts against phytopathogens for the first time, and provide the most comprehensive data on the antibacterial activity screening against food-borne pathogens. Considering limitations in plant disease control, antibacterial activities of these extracts would be important in plant disease control.
Fabrication of Monodispersive Nanoscale Alginate-Chitosan Core-Shell Particulate Systems For Controlled Release Studies
(2014) Korpe, Didem Aksoy; Malekghasemi, Soheil; Aydin, Ugur; Duman, Memed
Biopolymers such as chitosan and alginate are widely used for controlled drug delivery systems. The present work aimed to develop a new protocol for preparation of monodisperse alginate-coated chitosan nanoparticles at nanoscale. Modifications of preparation protocol contain changing the pH of polymer solutions and adding extra centrifugation steps into the procedure. While chitosan nanoparticles were synthesized by ionic gelation method, they were coated with alginate by electrostatic interaction. The size, morphology, charge, and structural characterization of prepared core-shell nanoparticulated system were performed by AFM, Zeta sizer, and FTIR. BSA and DOX were loaded as test biomolecules to core and shell part of the nanoparticle, respectively. Release profiles of BSA and DOX were determined by spectrophotometry. The sizes of both chitosan and alginate-coated chitosan nanoparticles which were prepared by modified protocol were measured to be 50 +/- 10 and 60 +/- 3 nm, respectively. After loading BSA and DOX, the average size of the particles increased to 80 +/- 7 nm. Moreover, while the zeta potential of chitosan nanoparticles was positive value, the value was inverted to negative after alginate coating. Release profile measurements of BSA and DOX were determined during 57 and 2 days, respectively. Our results demonstrated that monodisperse alginate-coated nanoparticles were synthesized and loaded successfully using our modified protocol.
Grafting Tomato Onto Tobacco Rootstocks Is A Practical and Feasible Application for Higher Growth and Leafing in Different Tobacco-Tomato Unions
(2016) Haberal, Mehmet; Korpe, Didem Aksoy; Iseri, Ozlem Darcansoy; Sahin, Feride Iffet; 0000-0002-3462-7632; 0000-0001-7308-9673; AAJ-8097-2021; AAC-7232-2020
A previously developed grafting strategy was applied to graft a commercial cultivated variety of tomato (Solanum lycopersicum L. H-2274) onto Nicotiana rustica L. (cv. Hasankeyf) and Nicotiana tabacum L. (cv. Samsun) rootstocks. Higher growth and leafing, and earlier flower onset were found in grafted than in non-grafted and self-grafted plants. Significant 22.7 and 34.3% increases in fruit yield were obtained with Samsun and Hasankeyf rootstocks, respectively. Leaves of tobacco-grafted plants had significantly elevated levels of nicotine, and highest leaf nicotine levels were measured at 90days after transplantation (DAT). Leaves from upper sampling levels of plants had significantly less nicotine independent of the sampling DAT. Fruits of all tobacco-grafted plants had significantly higher amounts of nicotine than non-grafted scions. Nicotine levels were higher in fruits sampled from lower parts of the plants for different harvest DAT and rootstocks. Increases in leaf nicotine concentration of tobacco-grafted plants were higher when compared with that of fruit. Grafting tomato onto tobacco rootstocks is a practical and feasible application for different tobacco-tomato unions.
High salt induced oxidative damage and antioxidant response in tomato grafted on tobacco
(2015) Iseri, Ozlem Darcansoy; Korpe, Didem Aksoy; Sahin, Feride Iffet; Haberal, Mehmet
One of the major limitations on agricultural development in many countries is the high salinity of the groundwater used in irrigation. Grafted plants may exhibit phenotypic variations from scion and rootstock plants in terms of abiotic stress tolerance, and be a method for improvement of tolerance in agricultural practices. The aim of the present study was to investigate response of Solanum lycopersicum L. ('Elazig') grafted on Nicotiana tabacum L. ('Samsun') and Nicotiana rustica L. ('Hasankeyf'), namely "Tomacco" plant (patent nr TR-2008-05391-B), to 10-d high NaCl irrigation. Physical development, chlorophyll a and b, total chlorophyll, total carotenoid, and anthocyanin levels were evaluated. Proline, lipid peroxidation, and electrolyte leakage levels were assayed in roots and leaves together with ascorbate peroxidase (APX) and catalase (CAT) activities. Considering alterations in chlorophyll contents, proline, malondialdehyde (MDA), and conductivity levels, and antioxidant enzyme activity levels scion and self-grafted plants seem to be more affected by salt treatments than tobacco and rootstock grafted plants. Tobacco roots seem to have better adaptive responses against salt stress in comparison to tomato as supported by changes in proline, APX, and CAT levels. Self-grafting experiments further supported grafting tomato onto tobacco rootstocks enhanced salt tolerance and adaptive response of scions and these changes seem to be dependent on rootstock rather than graft-induced changes. In conclusion, we demonstrated that previously defined graft unions of tomato on tobacco, which have increased fruit yield, had also enhanced tolerance to high salt stress and a promising technique for the cultivation of more salt tolerant varieties.
Hydrogen Peroxide Acclimation Enhanced Antioxidant Response of Lycopersicon Esculentum Mill. Under Prolonged Cold Stress: A Field Study
(2016) Iseri, Ozlem Darcansoy; Sahin, Feride Iffet; Korpe, Didem Aksoy; Haberal, Mehmet; 0000-0001-7308-9673; 0000-0002-3462-7632; AAC-7232-2020; AAJ-8097-2021