Browsing by Author "Keskin, D."

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    Multilayer fibroin/chitosan oligosaccharide lactate and pullulan immunomodulatory patch for treatment of hernia and prevention of intraperitoneal adhesion
    (2021) Akbaba, S.; Atila, D.; Keskin, D.; Tezcaner, T.; 0000-0002-3641-8674; 33966830; AAD-9865-2021
    This study aims to develop a novel intraperitoneal two- or three-layered patch with immunomodulatory property for treatment of hernia, regeneration of abdominal wall and prevention of intraperitoneal adhesions. Polypropylene (PP) mesh, middle layer, was intended to provide mechanical support whereas pullulan (PUL) hydrogel coating layer was designed to prevent intraperitoneal adhesions. Fibroin/chitosan oligosaccharide lactate (F/COS) layer electrospun on one side of pullulan was chosen for immunomodulation and abdominal wall regeneration. Physical and mechanical properties and regenerative capacity of intraperitoneal patches were determined. Immunomodulatory property of electrospun layer and whole patch was studied by determining nitric oxide amount produced by RAW 264.7 macrophages. 25 % (w/v) PUL hydrogel and F/COS with 90:10 (w/ w) ratio yielded optimal results. Here, we report that fabricated intraperitoneal patches successfully prevented cell adhesion on one side and increased cell viability and proliferation on other side, along with immunomodulation, in vitro.
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    Serum Angiostatin Levels in Patients with Behcet's Disease: Does Angiogenesis Play A Role in The Pathogenesis of Behcet's Disease?
    (2014) Keskin, D.; Keskin, G.; Inal, A.; Ozisik, L.; https://orcid.org/0000-0002-0690-2529; 25012748; J-8056-2012
    Angiogenesis plays an important role in the pathogenesis of inflammatory diseases, but the possible role of angiogenesis in Behcet's disease (BD) has not yet been studied. The aim of this study was to determine angiostatin levels in patients with BD and the role of angiogenesis in the pathogenesis of the disease. Thirty-seven patients with BD (mean age: 28.6 +/- 5.4 years, mean disease duration: 9.3 +/- 3.7 years) and 18 healthy controls were enrolled to the study. Twenty-four patients were in active and 13 patients were in inactive stage of the disease. The mean serum angiostatin level of patients with BD was 113.9 +/- 653.2 and 60.7 +/- 20.1 ng/ml in healthy controls. The mean serum angiostatin level was 142.7 +/- 43.1 ng/ml in active and 86.9 +/- 15.5 ng/ml in inactive patients with BD. Serum angiostatin levels were significantly high in patients with BD compared with healthy controls (P<0.001) and it was significantly high in active patients compared with inactive patients with BD (P<0.001). In inactive patients with BD, serum angiostatin concentrations were found to be higher compared with healthy controls (P<0.01). In active BD patients, the mean serum angiostatin level was correlated with the deep vein thrombosis (r=0.482, P=0.05), uveitis (r=0.582, P=0.01), and arthritis (r=0.492, P=0.05). According to these results; elevated serum angiostatin levels in patients with BD suggest the possible role of angiogenesis in the pathogenesis of the disease and its high levels in inactive Behcet's patients is related with the continuous activation of the disease even in the subclinical period.