Browsing by Author "Kayar, Nezahat Arzu"

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The Effects of Non-Surgical Periodontal Therapy on Neutrophil Elastase and Elastase Alpha-1 Proteinase Inhibitor Levels in GCF in Periodontitis Patients with or Without Acute Coronary Syndrome
(2021) Kayar, Nezahat Arzu; Ustun, Kemal; Gozlu, Muammer; Haliloglu, Seyfullah; Alptekin, Nilgun Ozlem; 33687554
Objective Periodontitis may contribute to vascular damage, resulting in the destabilization of atherosclerotic plaque leading to acute coronary syndrome (ACS). In this study, we explored the effect of non-surgical periodontal treatment (NSPT) on cardiovascular blood biomarkers and gingival crevicular fluid (GCF) neutrophil elastase (NE) and alpha 1-proteinase inhibitor (alpha-1PI) levels in periodontitis (P) participants with and without ACS. Materials and methods Medical and dental examinations were performed to diagnose ACS and periodontitis, respectively. Seventeen patients with diagnosis of ACS and periodontitis were included in this study, as a test group (group ACS). Twenty-six age and sex-matched control patients with periodontitis (group P) were otherwise systemically healthy. Both groups received NSPT. Plasma levels of cholesterol, triglyceride, high-density lipoprotein (HDL), low-density lipoprotein (LDL), C-reactive protein (CRP), GCF NE activity, GCF alpha(1)-PI levels, and GCF NE/alpha 1-PI rates were measured at baseline, at1(st) and 3(rd) months after NSPT. Results GCF NE activity/time (mu U/30s) decreased significantly at 3(rd) month compared to baseline values in the Group P after NSPT. First and 3(rd) months after NSPT, in the Group P GCF alpha(1)-PI activity/time (pg/30s) was significantly higher than the Group ACS. Moreover GCF NE/alpha(1)-PI rates decreased significantly compared to baseline values at 1(st) and 3(rd) months after NSPT in the group P. Conclusion NSPT yields decrease in NE/alpha(1)-PI rates. NE and its possible interactions with alpha(1)-PI may play a crucial role in both periodontitis and ACS. GCF alpha 1PI activity/time (U/30s) can be a potential biomarker in management of periodontitis associated with ACS.
Immune responses in women with periodontitis and preterm low birth weight: Levels of CD4+and CD8+T cells in gingival granulation tissue
(2020) Kayar, Nezahat Arzu; Celik, Ilhami; Alptekin, Nilgun Ozlem; 0000-0003-4104-6462; 31542704; G-1816-2014
Objective: Preterm Low-Birth-Weight (PLBW) is frequently associated with periodontal disease. However, the mechanism is still unknown. The present study was performed to examine the possible link between periodontal infections and PLBW in post-partum women utilizing clinical parameters and CD4+ and CD8 + T lymphocytes ratio in gingival granulation tissue. Materials: The tissues used in this study consisted of 35 gingival granulation tissue biopsies from 35 mothers of healthy infants (HTBW), 35 biopsies of gingival granulation tissue from 35 mothers of PLBW within one month postpartum and gingival tissue biopsies from 7 control individual with no periodontal disease (HC). CD4 + and CD8 + T lymphocyte ratios in a unit area of the gingival granulation tissue were determined by hystometrically. Statistical analysis was performed by using Kruskal-Wallis and Mann-Whitney U tests. Results: CD8 + T lymphocytes were more prevalent in the PLBW group than in the HTBW and HC group (P < 0.05). The CD4 + /CD8 + ratio in the PLBW group was lower than those of the other groups (p < 0.05). There were no statistically significant differences in CD4 + T lymphocytes counts between all groups (P > 0.05). Conclusion: Within the limits of this study it can be concluded that CD8 + T lymphocytes in gingival tissue may play important roles in the pathogenesis of periodontitis and PLBW.
Interleukin-1 Receptor Antagonist Gene Polymorphism, Adverse Pregnancy Outcome and Periodontitis in Turkish Women
(2015) Kayar, Nezahat Arzu; Alptekin, Nilgun Ozlem; Erdal, Mehmet Emin; 0000-0002-6191-2930; 0000-0003-4104-6462; 0000-0002-2325-2900; 26445016; F-9241-2015; G-1816-2014; AAA-7336-2021
Objective: The aim of this study was to determine associations between interleukin (IL)-1A (+4845), IL-1B (+3954), and IL-1 receptor antagonist (RN) variable number tandem repeat polymorphisms and adverse pregnancy outcomes and periodontitis in a Turkish women. Design: A total of 156 patients, including 64 women with normal birth outcome (NB) and 92 women with preterm/low birth weight outcome (PLBW) were included in this case-control study. Within 24 h after labor, maternal demographic characteristics and clinical periodontal parameters were recorded. The distribution and genotype frequencies of IL-1 were analyzed with polymerase chain reaction-restriction fragment length polymorphism assay. Statistical analyses were carried out for clinical periodontal parameters, genotype frequencies, and to identify explanatory variables for PLBW. Results: PLBW was associated with maternal age (p < 0.05), irregular prenatal care (p < 0.001), previous PLBW (p < 0.05), and antibiotic use during pregnancy (p < 0.05). Measurements of probing depth and clinical attachment level (CAL) were significantly higher in the PLBW group than in the NB group (p < 0.001). PLBW was associated with IL-1RN allele 2 (p < 0.001). Moreover, stepwise logistic regression analysis showed that CAL (OR 1.39, 95% CI: 1.04-1.85) and IL-1RN polymorphism (OR 7.92, 95% CI: 2.76-22.79), previous PLBW (OR 5.01, 95% CI: 1.08-23.17), age (OR 1.22, 95% CI: 1.04-1.44) were predictors found to increase the risk of PLBW (p < 0.05). There was a negative association between PLBW and regular prenatal care, total number of births, use an antibiotic during pregnancy period (p < 0.05). Conclusion: Our study showed that, IL-1RN allele 2, periodontal disease characterized with clinical attachment loss, previous PLBW and age could be an important risk factors for PLBW. (C) 2015 Elsevier Ltd. All rights reserved.
Methodological evaluation of gingival crevicular fluid volume and neutrophil elastase levels: sequential sampling, length of sampling time and two different sampling methods
(2019) Kayar, Nezahat Arzu; Oduncuoglu, Bahar Fusun; Haliloglu, Seyfullah; Serpek, Behic; Ataoglu, Tamer; Alptekin, Nilgun Ozlem; 0000-0003-0647-9481; 31852321; AAH-5405-2020; AAQ-4792-2020
Objectives: The mechanisms underlying the formation and composition of gingival crevicular fluid (GCF) and its flow into and from periodontal pockets are not understood very well. The aim of this study was to evaluate the length of sampling time and sequential sampling of GCF neutrophil elastase (NE) enzyme levels by using intracrevicular and orifice methods. Material and methods: Twenty adults (mean age of 41.8 years, ranged 31-60 years, 18 males and 2 females) with chronic periodontitis were enrolled and all completed the 3-d study. GCF was collected by both intracrevicular and intrasulcular methods, 720 samples of GCF were collected. In first, second and third day, the length of sampling time in seconds (s) and order were '5- 10-30-s'; '10- 30- 5-s' and '30- 5- 10-s,' respectively. GCF elastase levels were determined by hydrolysis of neutrophil specific substrate N-methoxysuccinyl-Ala-Ala-Pro-Val-p-nitroanilide. Results: NE activity (mu U) and NE activity/volume (mu U/mu l) were significantly different for order of sampling (p < .05), but not for the length of sampling time (p>.05). Conclusions: Within the limits of this study, the choice of sampling technique in GCF-profile studies seems to be a critical decision as it has the potential to affect the GCF volume and NE activity.
Systemic treatment with alpha-tocopherol and/or sodium selenite decreases the progression of experimental periodontitis
(2020) Bas, Nurgul; Kayar, Nezahat Arzu; Baba, Z. Fusun; Avunduk, Mustafa Cihat; Haliloglu, Seyfullah; Alptekin, Nilgun Ozlem; 32986166
Objective To investigate the effects of sodium selenite (Se) and/or alpha-tocopherol (alpha T) applications on the alveolar bone loss (ABL), the number of gingival collagen fibers, inducible nitric oxide synthase (iNOS)+ and CD95+ cell numbers, and serum cytokine concentrations in experimental periodontitis in rats. Materials and methods Forty Sprague Dawley rats were divided into four groups of ten as follows: group A: Se group, group B: alpha T group, group C: Se and alpha T combined group, and group D: control group (intraperitoneal (IP) saline injection applied). Using the image analysis method in the connective tissue under the connective epithelium, the numbers of iNOS, CD95 positive cells, and collagen fibers were counted. ELISA kits were used to test the concentrations of serum interleukin (IL)-1 beta, IL-6, and IL-4. Results The combination of Se and alpha T (group C) suppressed ABL compared with the control group (group D) (P< 0.05). In group A (Se), the number of iNOS+ cells was smaller than in group D (P< 0.05). Conclusion Se has been concluded to inhibit inflammation of the gum due to iNOS. Se and alpha T can have a remarkable important role in preventing alveolar bone loss, and particularly in combination.