Browsing by Author "Karahaliloglu, Zeynep"

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Potent bioactive bone cements impregnated with polystyrene-g-soybean oil-AgNPs for advanced bone tissue applications
(2019) Ilhan, Elif; Karahaliloglu, Zeynep; Kilicay, Ebru; Hazer, Baki; Denkbas, Emir Baki
Postoperative infection in orthopaedic and trauma surgery is one of the most feared complications. Recently, the high prevalence of multidrug-resistant bacteria has made the antibiotic treatment ineffective; thus novel non-antibiotic alternative approaches to this problem are urgently needed. Based on these expectations, in this work, in order to enhance the cytocompatibility and antibacterial performances of poly (methyl methacrylate (PMMA) and beta-tricalcium phosphate (beta-TCP) bone cements were impregnated with polystyrene (PS)-g-soybean oil graft copolymer containing AgNPs (PS-Agsbox), and we assessed the antimicrobial activity of the fabricated bone cements against Staphylococcus aureus and Escherichia coli. Nanoparticles at concentration of 1.25% (5 beta-TCP) w/w in beta-TCP bone cements were able to inhibit pathogens growth, while a concentration of 3.75% (15PMMA) was needed for PMMA bone cement. Therefore, the impregnated bone cements with PS-AgsboxNPs may be further explored as an alternative antimicrobial therapy for the treatment of infected bone defects.
The preparation of chitosan membrane improved with nanoparticles based on unsaturated fatty acid for using in cancer-related infections
(2020) Kizaloglu, Abdullah; Kilicay, Ebru; Karahaliloglu, Zeynep; Hazer, Baki; Denkbas, Emir Baki
This study includes the design of a chitosan membrane decorated with unsaturated fatty acid-based carrier system for cancer treatment and antibacterial application. For this, polystyrene-graft-polyoleic acid-graft-polyethylene glycol was prepared by free radical polymerization and characterized. Nanoparticles and caffeic acid-loaded nanoparticles were prepared by solvent evaporation technique and optimized. The short-term stability of nanoparticles was investigated at 4 degrees C. Drug encapsulation and loading efficiency were evaluated. The chitosan membrane and caffeic acid-loaded nanoparticles embedded into chitosan membrane were fabricated. The caffeic acid loaded nanoparticles embedded into chitosan membrane showed controlled release. The mechanical properties of all samples were investigated. The caffeic acid-loaded nanoparticles embedded into chitosan membranes indicated excellent antibacterial properties against theEscherichia coliandStaphylococcus aureus. The anticancer activity of all the samples was evaluated against SaOS-2 human primary osteogenic sarcoma and MC3T3-E1 pre-osteoblast cell lines by 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide assay, the flow cytometry and double staining methods. As a result, the designed carrier system showed great potential to cancer-associated infections treatment in bone cancer cases.
Prevention of urinary infection through the incorporation of silver-ricinoleic acid-polystyrene nanoparticles on the catheter surface
(2021) Koc, Hazal; Kilicay, Ebru; Karahaliloglu, Zeynep; Hazer, Baki; Denkbas, Emir B.; 33530824
Nosocominal infections associated with biofilm formation on urinary catheters cause serious complications. The aim of this study was to investigate the feasibility of the polyurethane (PU) catheter modified with tetracycline hydrochloride (TCH) attached Ag nanoparticles embedded PolyRicinoleic acid-Polystyrene Nanoparticles (PU-TCH-AgNPs-PRici-PS NPs) and the influence on antimicrobial and antibiofilm activity of urinary catheters infected by Escherichia coli and Staphylococcus aureus. For this purpose, AgNPs embedded PRici graft PS graft copolymers (AgNPs-PRici-g-PS) were synthesized via free radical polymerization and characterized by FTIR, HNMR and DSC. AgNPs-PRici-PS NPs were prepared and optimized by the different parameters and the optimized size of nanoparticle was found as about 150 +/- 1 nm. The characterization of the nanoparticles and the morphological evaluation were carried out by FTIR and SEM. Short term stability of nanoparticles was realised at 4 degrees C for 30 days. In vitro release profiles of TCH and Ag NPs were also investigated. The formation of biofilm on PU modified TCH-Ag NPs-PRici-PS NPs, was evaluated and the biocompatibility test of the nanoparticles was realized via the mouse fibroblast (L929) and mouse urinary bladder cells (G/G An1). This is the first time that TCH-AgNPs-PRici-PS NPs used in the modification of PU catheter demonstrated high antimicrobial and antibiofilm activities against the urinary tract infection.