Browsing by Author "Inal, Ali"

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Analysis of Panel Reactive Antibodies in Renal Transplant Recipients Detected by Luminex: A Single-Center Experience
(2016) Inal, Ali; Ozcelik, Umit; Uyanik, Ebru Ogan; Kulah, Eyyup; Demirag, Alp; 0000-0002-0690-2529; 0000-0003-1073-2494; 0000-0001-6041-4254; 26517205; J-8056-2012; AAG-8651-2021; AAJ-5764-2021
Objectives: The role of panel reactive antibody has gained universal acceptance in solid-organ transplant. This parameter is used to gauge the level of sensitization of prospective solid-organ recipients. More than one-third of patients on wait lists for kidney transplant are sensitized. Most have previously formed donor-specific and non-donor-specific serum antibodies and/or positive crossmatch by complementdependent cytotoxicity and/or flow cytometry. We present the rate of positivity at our institution for human leukocyte antigen antibodies and describe the condensation of antibodies in human leukocyte antigens for renal pretransplant recipients. Materials and Methods: Between January 2011 and December 2012, six hundred twenty consecutive renal transplant recipients on the wait list at the Baskent University were evaluated for this retrospective study. Panel reactive antibody screening and definition tests were studied with Luminex assays for the combination of class I (A, B, C) and class II antigens (DR, DQ). Results: We found a panel reactive antibody screening positivity in 20.4% of our patients on renal transplant waiting list. Panel reactive antibody defining tests were meaningful in 12.2% of the whole list. We observed that only panel reactive antibody class I positivity was seen in 2.2%, only panel reactive antibody class II positivity was seen in 2.7%, and both panel reactive antibody class I and class II positivities were seen in 7.2% of the defining tests. Conclusions: The estimated risk of sensitization for patients with a living donor is determined from the combined results of the crossmatch with the donor and those of the recipient's panel reactive and donorspecific antibodies. Compared with complementdependent cytotoxicity crossmatch, Luminex assays provide greater sensitivity and specificity in detection of donor-specific antibodies.
Immunology of Liver Transplantation
(2014) Inal, Ali; https://orcid.org/0000-0002-0690-2529; 24635783; J-8056-2012
In comparison with other solid-organ transplants, liver allografts are immunologically privileged. Allografts are rejected by immune reactions of the host, and clinical therapy for liver allografts includes immunosuppression to prevent rejection. Orthotopic liver transplant causes systemic donor-specific T-cell tolerance. In addition, antigens introduced into hepatocytes or the portal vein cause tolerance. The basic mechanism in liver tolerance may include continuous exposure of diverse liver cell types to endotoxin derived from intestinal bacteria. This exposure promotes the expression of cytokines, antigen-presenting molecules, and costimulatory signals that inactivate T cells, partly by effects on liver antigen-presenting cells. A simple, reliable, noninvasive assay to evaluate antidonor alloreactivity may be important in implementing these approaches in the laboratory and clinic.
INTERLEUKIN-27 IN TAKAYASU'S ARTERITIS: SERUM LEVELS AND RELATIONSHIP WITH DISEASE ACTIVITY
(2019) Keskin, Goksal; Inal, Ali; Cerci, Pamir; Olmez, Umit
Investigating the Role of IL-33 in the Pathogenesis of Behcet's Disease
(2017) Cerci, Pamir; Altiner, Seda; Inal, Ali; Kose, Kenan; Keskin, Goksal; Olmez, Umit; 0000-0002-0690-2529; 28412856; J-8056-2012
Objectives: Behcet's disease (BD) is an inflammatory disease, characterized by oral aphthous lesions, recurrent uveitis, skin lesions, and genital ulcerations. Increased release of several cytokines may play a role in the inflammatory stages of BD. IL-33, a member of the IL-1 cytokine superfamily, plays an important role in inflammation. We analyzed serum IL-33 concentration in BD patients to assess its possible role in the pathophysiology of this disease. Methods: The study included 54 BD patients, 31 with active BD and 23 with inactive BD as well as 18 matched healthy controls. Serum IL-33 levels were measured using an enzyme-linked immunosorbent assay (ELISA). Results: The mean serum IL-33 levels were 4.84 +/- 2.81 pg/ml in the BD patients (6.16 +/- 2.94 pg/ml in the active stage and 2.86 +/- 0.54 pg/ml in the inactive stage) and 2.88 +/- 0.42 pg/ml in the healthy controls. Serum IL-33 levels were significantly higher in patients with BD compared with the healthy controls (p < 0.01). In active Behcet patients with arthritis the mean serum IL-33 level was higher but this finding was not statistically significant (p = 0.122). Conclusion: IL-33 may play a significant role of in the pathogenesis of BD.
Investigating the Role of IL-33 in the Pathogenesis of Familial Mediterranean Fever
(2016) Keskin, Goksal; Tutluer, Seda; Inal, Ali; Cerci, Pamir; Olmez, Umit; 0000-0002-0690-2529; J-8056-2012
Our Experience with Paired Kidney Exchange Transplantation
(2018) Haberal, Mehmet; Akdur, Aydincan; Karakayali, Feza Yarbug; Ozcelik, Umit; Moray, Gokhan; Kulah, Eyyup; Inal, Ali; Torgay, Adnan; Arslan, Gulnaz; 0000-0002-3462-7632; 0000-0002-8726-3369; 0000-0002-1874-947X; 0000-0003-1073-2494; 0000-0003-2498-7287; 0000-0001-6041-4254; 0000-0002-0690-2529; 0000-0002-6829-3300; AAJ-8097-2021; AAA-3068-2021; AAB-3888-2021; AAG-8651-2021; AAE-1041-2021; AAJ-5764-2021; J-8056-2012; AAJ-5221-2021
Phagocytic Ability of Granulocytes and Monocytes in Elderly Patients with Community Acquired Pneumonia
(2018) Parmaksiz, Elif Torun; Inal, Ali; Caglayan, Benan; Dogan, Coskun; Fidan, Ali; Kiral, Nesrin; Comert, Sevda; 0000-0002-3670-8508; 0000-0002-0690-2529; GPX-1914-2022; J-8056-2012; HJB-3185-2022; HJG-7682-2022; HJH-4482-2023
The relationship of serum vitamin D receptor levels with disease activity and clinical parameters in patients with ankylosing spondylitis
(2019) Kultur, Turgut; Oztas, Dilek; Keskin, Dilek; Keskin, Goksal; Inal, Ali; Kara, Halil; 31893276
Objectives: The aim of this study was to investigate the relationship between serum vitamin D receptor (SVDR) levels and disease activity parameters in patients with ankylosing spondylitis (AS). Patients and methods: Between July 2016 and January 2017, a total of 62 patients (51 males, 11 females; mean age 36.5 +/- 12.8 years; range, 23 to 49 years) with AS and 32 healthy volunteers (25 males, 7 females; mean age 41.57 +/- 13.6 years; range, 26 to 48 years) were included in the study. The SVDR levels were measured using the enzyme-linked immunosorbent assay. Erythrocyte sedimentation rate (ESR) and serum C-reactive protein (CRP) levels were recorded. The Bath Ankylosing Spondylitis Disease Activity Index (BASDAI) scores were used to assess disease activity. Results: Although there was no significant difference between the patient and control groups (p=0.66), SVDR levels were significantly elevated in patients with active AS (BASDAI score >= 4) (p=0.01). The SVDR levels significantly increased in AS patients with peripheral joint involvement and enthesitis (p=0.01, p=0.05, respectively). The SVDR levels significantly elevated in patients treated with non-steroidal anti-inflammatory drugs, compared to those treated with biological agents and control group (p=0.01, p=0.03, respectively). The SVDR levels were positively correlated with the BASDAI, CRP and ESR in the patient group (p=0.01, r=0.751; p=0.01, r=0.75; p=0.01, r=0.81, respectively). Conclusion: Our study results suggest that serum SVDR levels are associated with the disease activity and clinical parameters in patients with AS. Based on these findings, SVDR level may be used as a marker of disease activity in AS.
Serum Glucocorticoid-Inducible Kinase-1(SGK1) Levels in Patients with Psoriatic Arthritis
(2018) Keskin, Goksal; Inal, Ali; Keskin, Esra Dilek; Olmez, Umit; https://orcid.org/0000-0002-0690-2529; J-8056-2012