Browsing by Author "Haliloglu, Seyfullah"

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Effect of Passive Tobacco Smoking Exposure on the Periodontal Status of Turkish Children
(2021) Alan, Raif; Gezgin, Onur; Korkut, Emre; Turkoglu, Sukriye; Celik, Esra; Haliloglu, Seyfullah
Objective: This study aimed to assess the effect of passive tobacco smoking (PTS) through the comparison of the periodontal status and the C-reactive protein (CRP) and cotinine levels in the biologic fluids in children who are exposed and unexposed to PTS. Methods: A total of 148 participants, whom had smokers (PTS-exposed, n=82) and non-smokers (PTS-unexposed, n=66) in their families, respectively, were included in the study. Gingival index, plaque index, probing depth, and bleeding on probing were recorded. Moreover, saliva, urine, and gingival crevicular fluid samples were collected. CRP and cotinine levels in the biological fluids were determined by the enzyme-linked immunosorbent assay method. Results: Both groups exhibited similar values for periodontal parameters and salivary CRP levels were higher in PTS-exposed group but not significant. The mean urinary cotinine level was significantly higher in children exposed to PTS than in unexposed children. Conclusions: There was no evidence for causative role of PTS in periodontal disease in this study. Longitudinal studies including large populations should be conducted to provide stronger evidence for the causative role of PTS in periodontal disease. Also, further epidemiological studies on the social context of smoking should be performed to improve the quality of life and lifespan of the society.
The Effects of Non-Surgical Periodontal Therapy on Neutrophil Elastase and Elastase Alpha-1 Proteinase Inhibitor Levels in GCF in Periodontitis Patients with or Without Acute Coronary Syndrome
(2021) Kayar, Nezahat Arzu; Ustun, Kemal; Gozlu, Muammer; Haliloglu, Seyfullah; Alptekin, Nilgun Ozlem; 33687554
Objective Periodontitis may contribute to vascular damage, resulting in the destabilization of atherosclerotic plaque leading to acute coronary syndrome (ACS). In this study, we explored the effect of non-surgical periodontal treatment (NSPT) on cardiovascular blood biomarkers and gingival crevicular fluid (GCF) neutrophil elastase (NE) and alpha 1-proteinase inhibitor (alpha-1PI) levels in periodontitis (P) participants with and without ACS. Materials and methods Medical and dental examinations were performed to diagnose ACS and periodontitis, respectively. Seventeen patients with diagnosis of ACS and periodontitis were included in this study, as a test group (group ACS). Twenty-six age and sex-matched control patients with periodontitis (group P) were otherwise systemically healthy. Both groups received NSPT. Plasma levels of cholesterol, triglyceride, high-density lipoprotein (HDL), low-density lipoprotein (LDL), C-reactive protein (CRP), GCF NE activity, GCF alpha(1)-PI levels, and GCF NE/alpha 1-PI rates were measured at baseline, at1(st) and 3(rd) months after NSPT. Results GCF NE activity/time (mu U/30s) decreased significantly at 3(rd) month compared to baseline values in the Group P after NSPT. First and 3(rd) months after NSPT, in the Group P GCF alpha(1)-PI activity/time (pg/30s) was significantly higher than the Group ACS. Moreover GCF NE/alpha(1)-PI rates decreased significantly compared to baseline values at 1(st) and 3(rd) months after NSPT in the group P. Conclusion NSPT yields decrease in NE/alpha(1)-PI rates. NE and its possible interactions with alpha(1)-PI may play a crucial role in both periodontitis and ACS. GCF alpha 1PI activity/time (U/30s) can be a potential biomarker in management of periodontitis associated with ACS.
Methodological evaluation of gingival crevicular fluid volume and neutrophil elastase levels: sequential sampling, length of sampling time and two different sampling methods
(2019) Kayar, Nezahat Arzu; Oduncuoglu, Bahar Fusun; Haliloglu, Seyfullah; Serpek, Behic; Ataoglu, Tamer; Alptekin, Nilgun Ozlem; 0000-0003-0647-9481; 31852321; AAH-5405-2020; AAQ-4792-2020
Objectives: The mechanisms underlying the formation and composition of gingival crevicular fluid (GCF) and its flow into and from periodontal pockets are not understood very well. The aim of this study was to evaluate the length of sampling time and sequential sampling of GCF neutrophil elastase (NE) enzyme levels by using intracrevicular and orifice methods. Material and methods: Twenty adults (mean age of 41.8 years, ranged 31-60 years, 18 males and 2 females) with chronic periodontitis were enrolled and all completed the 3-d study. GCF was collected by both intracrevicular and intrasulcular methods, 720 samples of GCF were collected. In first, second and third day, the length of sampling time in seconds (s) and order were '5- 10-30-s'; '10- 30- 5-s' and '30- 5- 10-s,' respectively. GCF elastase levels were determined by hydrolysis of neutrophil specific substrate N-methoxysuccinyl-Ala-Ala-Pro-Val-p-nitroanilide. Results: NE activity (mu U) and NE activity/volume (mu U/mu l) were significantly different for order of sampling (p < .05), but not for the length of sampling time (p>.05). Conclusions: Within the limits of this study, the choice of sampling technique in GCF-profile studies seems to be a critical decision as it has the potential to affect the GCF volume and NE activity.
Systemic treatment with alpha-tocopherol and/or sodium selenite decreases the progression of experimental periodontitis
(2020) Bas, Nurgul; Kayar, Nezahat Arzu; Baba, Z. Fusun; Avunduk, Mustafa Cihat; Haliloglu, Seyfullah; Alptekin, Nilgun Ozlem; 32986166
Objective To investigate the effects of sodium selenite (Se) and/or alpha-tocopherol (alpha T) applications on the alveolar bone loss (ABL), the number of gingival collagen fibers, inducible nitric oxide synthase (iNOS)+ and CD95+ cell numbers, and serum cytokine concentrations in experimental periodontitis in rats. Materials and methods Forty Sprague Dawley rats were divided into four groups of ten as follows: group A: Se group, group B: alpha T group, group C: Se and alpha T combined group, and group D: control group (intraperitoneal (IP) saline injection applied). Using the image analysis method in the connective tissue under the connective epithelium, the numbers of iNOS, CD95 positive cells, and collagen fibers were counted. ELISA kits were used to test the concentrations of serum interleukin (IL)-1 beta, IL-6, and IL-4. Results The combination of Se and alpha T (group C) suppressed ABL compared with the control group (group D) (P< 0.05). In group A (Se), the number of iNOS+ cells was smaller than in group D (P< 0.05). Conclusion Se has been concluded to inhibit inflammation of the gum due to iNOS. Se and alpha T can have a remarkable important role in preventing alveolar bone loss, and particularly in combination.