Browsing by Author "Gong, Weihua"

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    Introduction of Modified Cervical Cardiac Transplant Model in Mice
    (2012-04) Gong, Weihua; Balasubramanian, Savithri; Liu, Derek J.; Yuan, Shunzong; Ge, Fangmin; Whitcher, Gregory H.; Thornley, Thomas
    Objectives: The murine cervical heterotopic heart transplant model was initially designed to test the immune response to third-party allografts, modified by cuff techniques. While cuff techniques simplify the execution of this procedure, cutting of the carotid artery and the external jugular vein alters the blood supply to central nervous system and makes it difficult to achieve long-term graft survival. In the present study, we describe modified techniques that preserve the continuity and function of blood vessels and improve transplant integrity. Materials and Methods: The modified techniques in this study comprise the following aspects: (1) Preservation of the sternal head of the right sternocleidomastoid muscle, (2) use of the donor’s intrathoracic inferior vena cava for anastomosis and (3) preservation of the function of the recipient’s carotid artery and external jugular vein and thus, continuity of blood flow to the central nervous system. Results: Stable, long-term, disease-free allograft survival has been achieved with syngeneic transplants (> 200 days), whereas allografts from fully major histocompatibility complex-mismatched donors were acutely rejected in a time similar to the traditional abdominal heterotopic heart transplant model (8.2 ± 1.3 vs 8.4 ± 1.4 days; P = .73 in the Mantel Cox test, and P = .61 in the Gehan-Breslow-Wilcoxon test). Similar alloresponses could be induced in these 2 models. Conclusions: It is possible and feasible to achieve long-term graft survival in the mouse cervical heart transplant model using the modified procedures described in the present study.
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    Strategies For Successfully Establishing a Kidney Transplant in a Mouse Model
    (Başkent Üniversitesi, 2011-10) Ge, Fangmin; Gong, Weihua
    Objectives: Kidney transplant in mouse model is quite useful and valuable for studying transplant immunobiology. However, its technical complexity and high mortality have hindered widespread use. We sought to review and analyze the various techniques of kidney transplant in mice to prevent pitfalls and reduce complications. Materials and Methods: We searched PubMed using the key words “kidney transplant” or “renal transplant,” “mouse” or “mice,” and “technique” or “model” for articles published between January 1973 and June 2010. We found a series of factors that were collected and analyzed, which may influence establishing a mouse kidney transplant model. Results: A mouse kidney transplant model is practical for research. Mouse body weight, sex, animal strain, type of anesthesia, and type of flushing solution are relevant but do not determine a successful transplant. Warm and cold ischemia time should be less than 35 minutes and 2.5 hours. Conclusions: End-to-side vascular anastomosis and ureter-to-bladder or bladder-to-bladder for ureter reconstruction is preferred for establishing a kidney transplant model in mice.