Browsing by Author "Gocmen, J. Sedef"

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Comparison Of Efficacy And Reliability Of Six Commercial Covid-19 Diagnostic PCR Kits
(Başkent Üniversitesi Kazan Meslek Yüksek Okulu, 2024-03-26) Sharafi, Parisa; Gokdemir, F. Seyma; Akyol, Mesut; Ardicoglu-Akisin, Yasemin; Gocmen, J. Sedef; Taylan-Ozkan, Aysegul
Objectives. All countries have been deeply affected by the coronavirus disease 2019 pandemic, both economically and in situations that strain health systems, such as workforce and workload. Therefore, various measures should be taken to control the disease and prevent its spread. Since the disease onset, real-time PCR tests have been used as the gold standard for disease diagnosis. Owing to the rapid progress of the pandemic and the spread of the disease, validation, consistency, and optimization tests of some commercial kits have been conducted directly in the field. Therefore, it is important to compare the results of these kits and improve the existing ones. Methods. We compared five kits (Bioexen, Polgen, Coronex, Diagen, and Anatolia) donated to the TOBB Economics and Technology University Hospital PCR laboratory with the KrosGen kit to detect severe acute respiratory syndrome coronavirus 2. A total of 244 samples were selected and analyzed using five different severe acute respiratory syndrome coronavirus 2 PCR detection kits. Results. Positive and negative results from the six kits were compared using the working protocols of the kits, primers, and cycle threshold (Ct) values. Five of the six kits have reliable compatibility for Ct<30 but decreases for Ct >= 30. Therefore, it is important to evaluate the performance of these kits for reduced viral loads. Conclusions. Using a suitable kit with high compatibility for Ct >= 30 is important for detecting patients with a low viral load and helping prevent disease spread.
In Vitro Evaluation of E. coli And S. epidermidis Adhesion on Dressing Materials Used in Hypospadias Repair
(2014) Soyer, Tutku; Bostanoglu, Ebru; Aslan, Mustafa Kemal; Boybeyi, Ozlem; Demirbilek, Muge; Gocmen, J. Sedef; JWP-3001-2024
Background An in vitro study was performed to evaluate the adherence of Escherichia coli and Staphylococcus epidermidis to different dressing materials. Methods Hydrophyl gauze swab (GS), sterile flax swab (FS), and their nitrofurazone (Furacin (R)) absorbed forms (GSF and FSF), sterile paraffin-covered gauze with chlorhexidine acetate (0.5 %) (Bactigrass (R), BG), and semipermeable transparent film gauze (Tegaderm (R), TG) were used in the study. A 1 cm(2) of each material was placed in culture plates, and E. coli ATCC 25922 and S. epidermidis ATCC 12228 were suspended in each plate. After 24, 48 h, and 1 week of incubation period, colony counts were calculated in colony-forming units (CFU) per square centimeter unit. Bacterial adhesion was compared between groups, and each dressing material was also compared for different incubation periods (24, 48 h, and 1 week). Results The colony counts were significantly decreased in GSF and FSF groups (Kruskal-Wallis test, p < 0.05). There was no difference for E. coli adhesion in GS, FS, BG, and TG groups (p > 0.05). The significant difference detected for S. epidermidis adhesion in the first 24 to 48 h could not be detected at the end of a 1-week period. There was no statistical difference in groups when compared for 24-, 48-h, and 1-week periods of incubations (Wilcoxon signed-rank test, p > 0.05). In BG group, E. coli adhesion was increased, and S. epidermidis adhesion was decreased without a significant difference at the end of a 1-week period (p > 0.05). Conclusions Nitrofurazone absorbed sterile gauze materials have less bacterial adhesion. In vitro adhesion of E. coli and S. epidermidis did not differ between the 24-h and 1-week period. E. coli adhesion to BG can be increased if the duration of dressing prolonged up to a 1-week period. Level of Evidence: Not ratable.