Browsing by Author "Ergin, Makbule"

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Antioxidant Enzymes and Lipid Peroxidation in Cold Ischemic Lung Preservation
(Başkent Üniversitesi, 2009-06) Yeginsu, Ali; Ergin, Makbule
Objectives: Our purpose was to investigate antioxidant enzymes and lipid peroxidation in time course ischemic lung preservation in rats. Materials and Methods: Thirty-six Wistar rats were divided into 6 groups of 6 rats each. After having been anesthetized, the rats were intubated and connected to a rodent ventilator. Lung-heart blocks were excised. In the control group, the lungs were immediately stored at –80°C after removal. The lungs from the other groups were preserved in 40 mililiters of low potassium dextran solution at 4°C for 6, 12, 24, 48, and 72 hours, respectively. Antioxidant enzyme activity and malondialdehyde levels were then measured. Results: Superoxide dismutase activity significantly increased at the 12th hour and remained higher up to the 72nd hour (P < .001). Glutathione peroxidase activity was higher than that in the control group from the 6th to the 24th hour but was significant only at the 12th hour (P < .001) and decreased below the level in the control group after the 48th hour. Catalase activity was significantly higher than that in the control group in all preservation periods (P < .001). The nitric oxide level slowly increased and reached a significantly higher level than that in the control group at the 24th and 72nd hours (P = .028) and then decreased to the level found in the control group. The malondialdehyde level slightly increased from the 6th to the 24th hour, but that increase, when compared with the level in the control group, had no statistical significance (P = .110). Conclusions: In ischemic lung preservation, oxidative stress begins during the early phase of preservation and continues for up to 72 hours. Although oxidative stress continues for a significant period, an antioxidant mechanism adequately prevents its harmful effects on the lung. Thus no significant lipid peroxidation occurred in long-term ischemic lung preservation in the murine model studied.
Trauma Does Not Aggravate Deleterious Effects of Ischemia Reperfusion Injury on the Lung
(Başkent Üniversitesi, 2010-03) Ergin, Makbule; Caglar Goktas, Guleser; Akbas, Ali; Elmas, Cigdem; Ozyurt, Huseyin; Yeginsu, Ali
Objectives: Our purpose was to investigate the effects of ischemia-reperfusion injury on traumatized lungs. Materials and Methods: Twenty-four Wistar rats were used in the study. Rats were randomly divided into 4 groups. In the control group (group 1), only anesthesia and ventilation were used. In group 2, only lung ischemia-reperfusion injury was instituted. In group 3, only blunt chest trauma was instituted. And in group 4, lung ischemia-reperfusion injury, consisting of 24 hours after the constitution of blunt chest trauma, was used. Lung damage and systemic inflammation parameters were evaluated. Results: All parameters (alveolar degeneration grades, alveolar macrophage and lymphocyte counts, antioxidant enzyme activities, cytokine levels, and bronchoalveolar lavage fluid albumin level) were higher in all groups than they were in the control group (P < .05). Only tissue malondialdehyde, tissue interleukin-8, and serum glutathione peroxidase levels were significantly higher in the lung ischemia-reperfusion group than they were in the trauma group (P < .05). The trauma + ischemia-reperfusion group showed no significant difference when compared with the only ischemia-reperfusion or only trauma groups in any parameters (P > .05). Conclusions: The findings showed that lung trauma does not aggravate the deleterious effects of lung ischemia-reperfusion injury.