Browsing by Author "Efe, Oguzhan Ekin"

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Effect of rosuvastatin on spatial learning, memory, and anxiety-like behaviour in ovariectomized rats
(2022) Emre-Aydingoz, Selda; Lux, Karl Michael; Efe, Oguzhan Ekin; Topcu, Deniz Ilhan; Erdem, Saban Remzi; 0000-0001-7823-7620; 0000-0002-3243-7843; 0000-0002-1219-6368; 35993621; ABA-4291-2020; W-7908-2019; E-3717-2019
The effect of rosuvastatin (Ros) on cognitive function and anxiety-like behaviour in ovariectomized rats were evaluated. Eighteen female Wistar rats (218-310g, 6-8 months old) were allocated into sham (n = 6), ovariectomy (Ovx, n = 6) or Ovx + Ros (up to eighth week n = 6, then n = 4) groups. Ros was administered at 20 mg/kg/day by oral gavage for 12 weeks. Behavioural tests were performed at 4, 8 and 12 weeks following Ovx. At 12weeks, Ovx group had significantly longer escape latency than the sham group at the first day of the four-day training period of the Morris Water Maze test (p < .01). In the Elevated Plus Maze test, Ovx group spent significantly more time in the closed arms than the sham group (p < .01), and this anxiety-like behavioural effect of Ovx was prevented by 12-weeks Ros treatment (p < .05). In conclusion, Ros prevents memory deficit and anxiety-like behaviour in the ovariectomized rats, a model for human surgical menopause.
Effects of Hypoxia Versus Ischaemia on Vascular Functions of Isolated Rat Thoracic Aorta: Revisiting the in Vitro Vascular Ischaemia/Reperfusion Model
(2023) Orhan, Halit Guner; Teimoori, Ariyan; Demirtas, Elif; Zeynalova, Nargiz; Efe, Oguzhan Ekin; Aydingoz, Selda Emre; 37937174
Introduction The in vitro rat vascular ischaemia and reperfusion model is used to evaluate the molecular and functional effects of potential agents against ischaemia and reperfusion injury of autologous graft veins. However, there is no consensus on whether hypoxia, rather than ischaemia, is sufficient to induce vascular dysfunction. Aim To compare the effects of hypoxia and ischaemia, with or without reperfusion, on the vascular functions of isolated thoracic aortic rings of rats. Material and methods Thoracic aortas of 12 male Sprague-Dawley rats (350-500 g, 18-24 months old) were isolated and divided into rings that were randomly allocated to control, ischaemia, hypoxia, ischaemia-reperfusion, and hypoxia-reperfusion groups. Aortic rings other than those of the control group were stored at 4 degrees C for 24 h in saline. For ischaemia, saline was gassed with nitrogen. After 24 h, aortic rings in the ischaemia-reperfusion and hypoxia-reperfusion groups were incubated with 200 mu M sodium hypochlorite for 30 min. Vascular and endothelial functions were tested in an organ bath set-up. Results Vascular response to potassium chloride (80 mM) decreased in all experimental groups compared to the control group (p = 0.007), but phenylephrine-induced contraction (10-5 M) increased only in the ischaemia-reperfusion group (p < 0.0001). Acetylcholine (10-11-10-5 M)-induced endothelium-dependent vasorelaxations were impaired in all groups - particularly in the ischaemia-reperfusion group (p = 0.0011). Sodium nitroprusside (10-12-10-7 M)-induced endothelium-independent vasorelaxations were similar across all groups (p = 0.1258). Conclusions Ischaemia followed by reperfusion should be implanted to achieve maximum endothelial and contractile dysfunction in vitro, and to replicate ischaemia and reperfusion injury of autologous graft veins.
Effects of ozone therapy and taurine on ischemia/reperfusion-induced testicular injury in a rat testicular torsion model
(2014) Aydos, Tolga Resat; Basar, Mehmet Murad; Kul, Oguz; Atmaca, Hasan Tank; Uzunalioglu, Tuba; Kisa, Ucler; Efe, Oguzhan Ekin; 25539540
Background/aim: To investigate the effect of ozone and/or taurine treatment comparatively on testicular damage due to ischemia/reperfusion (I/R) injury in an experimental torsion model in rats. Materials and methods: Adult Wistar rats with and without torsion/detorsion were used. In order to monitor the effect of ozone and/or taurine treatment on testicular damage due to I/R injury, following histopathological investigation apoptotic indexes were scored by TUNEL method. Moreover, tumor necrosis factor receptor 1 (TNFR1), caspase 3, caspase 8, endothelial nitric oxide synthase (eNOS), tumor necrosis factor alpha (TNF alpha), and cytochrome C immunostainings were performed and the levels of malondialdehyde, glutathione peroxidase, superoxide dismutase, catalase, total sulthydryl, and nitric oxide were determined in the testicular tissue. Results: Intraperitoneal ozone and/or taurine treatment prevented both histopathological damage and increase in the apoptotic index. Torsion did not exert an effect on the levels of TNFa and cytochrome C. Ozone and/or taurine treatment prevented increases in TNFR1, caspase 3, and caspase 8. The level of oxidative stress markers was unchanged. The increases in NO level and eNOS expression were prevented by ozone and/or taurine treatment in I/R groups. Conclusion: Using ozone therapy and/or taurine before reperfusion may be a solution for germ cell degeneration resulting from testicular torsion and related infertility.
Mechanism of acitretin-induced relaxations in isolated rat thoracic aorta preparations
(2022) Efe, Oguzhan Ekin; Aydos, Tolga Resat; Aydingoz, Selda Emre; 0000-0002-3243-7843; 0000-0001-7823-7620; 34411501; W-7908-2019; ABA-4291-2020
Acitretin is a member of vitamin A-derived retinoids, and its effect on vascular smooth muscle had not yet been studied. The aim of this study is to investigate the effect of acitretin, a retinoid, on vascular smooth muscle contractility. Thoracic aorta preparations obtained from 34 male Sprague-Dawley rats (355 +/- 15 g) were studied in isolated organ baths containing Krebs-Henseleit solution. The relaxation responses were obtained with acitretin (10-12-10-4 M) in endotheliumpreserved and endothelium-denuded aorta preparations precontracted with submaximal concentration of phenylephrine (10-6 M). The role of retinoic acid receptors (RARs), nitric oxide, adenylyl, and guanylyl cyclase enzymes, and potassium channels in these relaxation responses were investigated. Acitretin produced concentration-dependent relaxations, which were independent of its solvent dimethylsulfoxide (DMSO), in endothelium-denuded phenylephrine-precontracted thoracic aorta preparations. While incubation with the RAR antagonist (AGN193109, 10-5 M) had no effect on these relaxations; nitric oxide synthase inhibitor (L-NG-Nitro arginine methyl ester (L-NAME), 10-4 M), adenylyl cyclase inhibitor (SQ2253, 10-5 M), guanylyl cyclase inhibitor (oxadiazolo [4,3-a] quinoxalin-1-one (ODQ), 10-6 M), and potassium channel blocker (tetraethylammonium (TEA), 10-2 M) significantly eliminated the relaxation responses induced by acitretin. Acitretin induces relaxation in rat isolated thoracic aorta preparations without endothelium, which may be mediated by nitric oxide, cyclic adenosine monophosphate, and cyclic guanosine monophosphate-dependent kinases and potassium channels.
POLYPHARMACY PREVALENCE AMONG GERIATRIC PATIENTS IN PRIMARY HEALTHCARE SETTINGS ACROSS TURKEY: A CROSS-SECTIONAL ANALYSIS THROUGH THE NATIONWIDE PRESCRIPTION INFORMATION SYSTEM
(2020) Aydos, ToIga Resat; Emre Aydingoz, Selda; Lux, Karl Michael; Efe, Oguzhan Ekin; Isli, Fatma; Aksoy, Mesil; Kadidoi, Esma; 0000-0002-3243-7843; 0000-0002-1832-9336; 0000-0001-7823-7620; W-7908-2019; AAJ-7279-2020; ABA-4291-2020
Introduction: Polypharmacy has become a common health problem as populations age. We aimed to determine the prevalence of chronic and cumulative polypharmacy in the geriatric population using primary healthcare services in Turkey. Materials and Methods: The electronic prescriptions ordered by family physicians across Turkey for geriatric patients (>= 6.5 years) in the Prescription Information System during 2018 were studied. Chronic polypharmacy criteria were proportion of patients who were given prescriptions containing >= 5 drugs four or more times during a year. Cumulative polypharmacy was defined as proportion of patients who were prescribed >= 5 drugs with different ATC4 codes in a month or in each quarter of the year. Results: Turkey's total population is 82 million; 7,186,204 are aged 65 and over, constituting 8.8% of the total. Of this geriatric population, 6,104,798 (85.0%) had at least one prescription in 2018. Each geriatric patient had 6.4 prescriptions, with each prescription containing an average of 2.9 drugs with different fourth-level Anatomical Therapeutic Chemical codes. Each drug was prescribed in 2.7 boxes on average. Of these prescribed patients, 14.3% received prescriptions containing >= 5 drugs four or more times during 2018. The percentage of patients who received at least one prescription per month containing >= 5 drugs ranged between 16.4% and 20.7%. The most commonly prescribed drugs were acetylsalicylic acid, diclofenac, paracetamol, and pantoprazole. Conclusion: Polypharmacy is a critical health problem among geriatric population in Turkey as in other industrialized countries. Educating physicians as well as the public is essential to overcome polypharmacy.
Reporting quality of animal studies published in journals listed in ULAKBIM TR index: a systematic review on compliance to the ARRIVE guidelines
(2022) Aydingoz, Selda Emre; Efe, Oguzhan Ekin; Caliskan, Gokce; 0000-0001-7823-7620; 0000-0002-3243-7843; ABA-4291-2020; W-7908-2019
Transparent reporting of animal studies is key to ensure reproducibility. The primary guideline for reporting animal studies is the ARRIVE (Animal Research: Reporting of In Vivo Experiments). We aimed to evaluate the compliance to the updated ARRIVE guidelines (version 2.0, date 2020) in animal studies published in journals indexed by the Turkish Academic Network and Information Centre (ULAKBIM TR Index). This was a retrospective analysis of reporting quality of animal studies published in journals listed in ULAKBIM TR Index between January 2010 and August 2021. The percentage of articles that fully reported each " of the ARRIVE Essential 10: Compliance Questionnaire was calculated, and effects of journal- and article-related factors were evaluated. Totally, 235 articles published in 89 journals were included into the analysis. The mean percentage of fully reported items was 59.1%+/- 10.9%, the least reported items being those related with bias - "blinding", "allocation to study groups", "assessment of statistical assumptions", "excluded animals", and "sample size calculation" (0.9%-10.6%). The journal's publisher, frequency, language, being indexed in the Science Citation Index Expanded, and the ratio of animal studies had no significant effect on this percentage (p>0.05). However, journals supporting the ARRIVE guidelines had significantly and higher compliance (62.1 %+/- 10.1% vs. 58.2%+/- 11.0% for supporters and non-supporters, respectively; p=0.017, 95%CI -7.0 to-0.7). Articles published after 2015 had higher compliance to the ARRIVE guidelines than those published previously (60.9%+/- 10.4% vs. 57.9%+/- 11.1%, respectively; p=0.037, 95%CI -5.8 to -0.2). Although progress has been made, compliance to the ARRIVE guidelines is still low in animal studies published in journals listed in ULAKBIM TR Index-Turkey's scientific journal database that covers over 800 peer-review journals. In order to increase the reporting quality and reproducibility of animal studies, it is imperative to raise awareness among researchers and journals, and to enforce the ARRIVE guidelines in editorial policy of journals.