Browsing by Author "Coskun, Erhan"

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The Effect of Adipose Derived Stromal Vascular Fraction on Stasis Zone in An Experimental Burn Model
(2018) Eyuboglu, Atilla Adnan; Uysal, Cagri A.; Ozgun, Gonca; Coskun, Erhan; Ertas, Nilgun Markal; Haberal, Mehmet; 0000-0002-3462-7632; 0000-0001-6236-0050; 0000-0002-9805-9830; 29032969; AAJ-8097-2021; AAJ-2949-2021; IQW-7940-2023
Background: Stasis zone is the surrounding area of the coagulation zone which is an important part determining the extent of the necrosis in burn patients. In our study we aim to salvage the stasis zone by injecting adipose derived stromal vascular fraction (ADSVF). Methods: Thermal injury was applied on dorsum of Sprague-Dawley rats (n=20) by the "comb burn" model as described previously. When the burn injury was established on Sprague-Dawley rats (30min); rat dorsum was separated into 2 equal parts consisting of 4 burn zones (3 stasis zone) on each pair. ADSVF cells harvested from inguinal fat pads of Sprague-Dawley rats (n=5) were injected on the right side while same amount of phosphate buffered saline (PBS) injected on the left side of the same animal. One week later, average vital tissue on the statis zone was determined by macroscopy, angiography and microscopy. Vascular density, inflammatory cell density, gradient of fibrosis and epithelial thickness were determined via immunohistochemical assay. Results: Macroscopic stasis zone tissue viability (32 + 3.28%, 57 + 4.28%) (p<0.01), average number of vessels (10.28 +/- 1.28, 19.43 +/- 1.72) (p<0.01), capillary count (15.67 +/- 1.97, 25.35 +/- 2.15) (p<0.01) vascular density (1.55 +/- 0.38, 2.14 +/- 0.45) (p<0.01) epithelial thickness (0.014 +/- 0.009mm, 0.024 +/- 0.0011mm) were higher on ADSVF side. Fibrosis gradient (1.87 +/- 0.51, 1.50 +/- 0.43) (p<0.01) and inflammatory cell density (1.33 +/- 0.40, 1.20 +/- 0.32) (p<0.01) were higher on the PBS side. Conclusion: Macroscopic and microscopic findings determined that ADSVF has a statistically significant benefit for salvaging stasis zone on acute burn injuries. (c) 2017 Elsevier Ltd and ISBI. All rights reserved.
The Effect of Adipose Stromal Vascular Fraction on the Viability of Transverse Rectus Abdominis Myocutaneous Flap after Abdominoplasty: An Experimental Study
(2021) Coskun, Erhan; Ozkan, Burak; Terzi, Aysen; Ozer, Eda Ozturan; Uysal, Cagri Ahmet; Borman, Huseyin; Ertas, Nilgun Markal; 0000-0002-1225-1320; F-7546-2013
Introduction: A prior abdominoplasty is considered as an absolute contraindication to transverse rectus abdominis musculocutaneous (TRAM) flap surgery. The aim of this study is to investigate the effect of nonexpanded adipose stromal vascular fraction (ASVF) on the viability of TRAM flap after abdominoplasty. Materials and Methods: Thirty-five male Sprague Dawley rats were divided into five groups. Reverse abdominoplasty model was used in all groups except Group 1. TRAM flap was performed 2 weeks after abdominoplasty in Groups 2 and 4 and 4 weeks after in Groups 3 and 5. ASVF cells were injected during abdominoplasty in Groups 4 and 5. The viable flap area (VFA) percentage and newly formed perforators were assessed. Capillary density and fibrosis gradient and plasma vascular endothelial growth factor (VEGF) levels were measured. Results: The mean VFA to total flap area was measured as 82.90% +/- 7.59%, 3.31% +/- 3.29%, 9.40% +/- 6.18%, 31.92% +/- 9.29%, and 64.98% +/- 10.95% in Group 1, Group 2, Group 3, Group 4, and Group 5, respectively (P < 0.05). The number of newly formed musculocutaneous perforating arteries was 0.29 +/- 0.49, 1.14 +/- 0.69, and 2 +/- 0.82 for Groups 3, 4, and 5, respectively (P < 0.05). The mean capillary density was 6.86 +/- 0.50, 0.67 +/- 0.13, 2.79 +/- 0.53, 3.71 +/- 0.47, and 7.01 +/- 0.70 in Groups 1, 2, 3, 4, and 5, respectively (P < 0.05). There was a statistically significant increase between the baseline VEGF values and the second VEGF values in Groups 4 and 5. Conclusions: The study showed that local injection of ASVF increases the viability of TRAM flap after abdominoplasty.