Browsing by Author "Bacanli, Didem"

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Antiproliferative and anti-apoptotic effect of astaxanthin in an oxygen-induced retinopathy mouse model
(2019) Kucukoduk, Ali; Helvacıoglu, Fatma; Haberal, Nihan; Dagdeviren, Atilla; Bacanli, Didem; Yilmaz, Gursel; Akkoyun, Imren; 0000-0001-8990-8282; 30851776; P-2877-2014
Objective: To evaluate the impact of intravitreal (IV) and intraperitoneal (IP) astaxanthin (AST) injections on neovascular development (ND), retinal morphology, and apoptotic activity in a C57BL/6J mouse model with hyperoxia-induced retinopathy (HIR). Design: C57BL/6J mouse model. Methods: Two negative control groups (n = 6 each; one of which received IV sterile dimethyl sulfoxide [DMSO]) of C57BL/6J-type mice were exposed to room air. The HIR groups included 36 C57BL/6J-type mice exposed to 75% +/- 2% oxygen from postnatal day (PD) 7 to PD 12. On PD 12, these mice were randomized into 6 groups (n = 6 each): 2 HIR control groups (one of which received IV-DMSO), 2 IV-AST groups (10 and 100 mu g/mL), and 2 IP-AST groups (0.5 and 5 mg/kg). We measured ND by counting neovascular tufts in cross sections and examined histological, ultrastructural changes via light and electron microscopy. Apoptosis was detected using terminal deoxynucleotidyl transferase-mediated nick end-labeling. Results: No ND was detected in the negative control groups. ND levels were not significantly different between high- and low-dose AST for either means of administration. However, ND levels were significantly lower in the AST groups, regardless of delivery, compared to the control groups. The means of delivery (IP versus IV) also yielded significant differences in ND. The incidence of mitochondrial dysmorphology and apoptosis were lower in groups receiving AST. Conclusions: AST seems to suppress ND and has anti-apoptotic activity in the HIR mouse model.
Antiproliferative and Mitochondrial Protective Effects of Apigenin in an Oxygen-Induced Retinopathy In Vivo Mouse Model
(2021) Sezenoz, Almila Sarigul; Akkoyun, Imren; Helvacioglu, Fatma; Haberal, Nihan; Dagdeviren, Attila; Bacanli, Didem; Yilmaz, Gursel; Oto, Sibel; 0000-0002-2860-7424; 34665015; AAK-7713-2021
Purpose: To investigate the effects of a common dietary flavonoid apigenin on retinal endothelial cell proliferation, retinal morphological structure, and apoptotic cell death in an oxygen-induced retinopathy (OIR) mouse model to evaluate the possibility of the use of apigenin in the treatment of ocular neovascular diseases (ONDs). Methods: Ninety-six newborn C57BL/6J mice were included. Eight groups were randomized, each including 12 mice. Two negative control groups were kept in room air: the first without any injection and the second received intravitreal (IV) dimethyl sulfoxide (DMSO), which is the solvent we used. The OIR groups were exposed to 75% +/- 2% oxygen from postnatal days (PD) 7 to 12. On PD 12, the mice were randomly assigned to 6 groups: 2 OIR control groups (1 received no injection, 1 received IV-DMSO), 2 IV-apigenin groups (10 and 20 mu g/mL), and 2 intraperitoneal (IP)-apigenin groups (10 and 20 mg/kg). We quantified retinal endothelial cell proliferation by counting neovascular tufts in cross-sections and examined histological and ultrastructural changes through light and electron microscopy. We evaluated apoptosis by terminal deoxynucleotidyl transferase-mediated nick end-labeling (TUNEL). Results: We detected a significant increase in endothelial cell proliferation in the OIR groups. Groups receiving apigenin, both IP and IV, had significant decreases in endothelial cells, atypical mitochondrion count, and apoptotic cells compared with the groups receiving no injections. None of the apigenin-injected groups revealed cystic degeneration or cell loss. Conclusions: Apigenin suppresses neovascularization, has antiapoptotic and antioxidative effects in an OIR mouse model, and can be considered a promising agent for treating OND. Clinical trial (Project number: DA15/19).
Bridging Technique of Bile Duct Anastomosis Using An Expanded Polytetrafluoroethylene (Eptfe) Graft in A Porcine Model
(2016) Haberal, Mehmet; Ozdemir, Handan; Bacanli, Didem; Ozcay, Necdet; Ersoy, Zeynep; Torgay, Adnan; https://orcid.org/0000-0002-3462-7632; https://orcid.org/0000-0003-0767-1088; AAJ-8097-2021; AAF-3066-2021
EFFECT OF NEW BASKENT UNIVERSITY ORGAN-PRESERVATION SOLUTION ON THE ACTIN CYTOSKELETON REARRANGEMENT AND APOPTOSIS OF RENAL TUBULAR CELLS: COMPARISON WITH OTHER PRESERVATION SOLUTIONS
(2019) Haberal, Mehmet; Ozdemir, B. Handan; Kirnap, Mahir; Erdem, Remzi; Lux, K. Michael; Bacanli, Didem; AAH-9198-2019
Effect of Rat Urine on Ileum Muscle Layer of Bladder Reconstruction: An Experimental Study
(2018) Ersoz, Dilsah; Kirnap, Mahir; Ozdemir, B. Handan; Bacanli, Didem; Haberal, Mehmet; https://orcid.org/0000-0002-7528-3557; https://orcid.org/0000-0002-3462-7632; AAH-9198-2019; X-8540-2019; AAJ-8097-2021
Effect of Rat Urine on Ileum Muscle Layer of Bladder Reconstruction: An Experimental Study
(2018) Ersoz, Dilsah; Kirnap, Mahir; Ozdemir, B. Handan; Bacanli, Didem; Haberal, Mehmet; 0000-0002-7528-3557; 0000-0002-3462-7632; AAH-9198-2019; X-8540-2019; AAJ-8097-2021
The Effects of Glucose and Fructose on Body Weight and Some Biochemical Parameters in Rats
(2018) Koseler, Esra; Kiziltan, Gul; Turker, Perim Fatma; Saka, Mendane; Ok, Mehtap Akcil; Bacanli, Didem; Aydos, Tolga Resat; Bayraktar, Nilufer; Ozdemir, Handan; 0000-0002-4254-3711; 0000-0002-1832-9336; 0000-0002-7886-3688; AAZ-8170-2020; AAJ-7279-2020; Y-8758-2018
Objective: Dietary fructose from added sugar as high fructose corn syrup may causes major risks in obesity, hyperlipidemia, cardiovascular diseases, hyperuricemia and fatty liver. The aim of this study was to investigate and compare the effects of high fructose and high glucose intake on body weight and some biochemical parameters in rats. Subject and methods: The study was conducted on adult, 32 Wistar albino male rats (300-350 g weeks) which fed with standard laboratory chow. In each group, 8 rats was selected randomly and which was be composed four groups. The rats in each group, in addition to standard meal, different amount of glucose and fructose containing solutions (10% and 30% glucose-fed group, 10% and 30% fructose-fed group) was given by oral gavage for 6 weeks. At baseline and after 6 weeks total cholesterol, VLDL-cholesterol, triglycerides, uric acid, AST and ALT as biochemical parameters and liver histopathological examination of rats were determined. Body weight of the rats was evaluated every week. Results: The 30% fructose group caused higher AST levels according to 10% glucose group, 30% glucose group and 10% fructose group. At the end of 6 weeks, the mean body weight in the fructose-fed groups was higher than the glucose-fed groups (p>0.05). No statistically significant difference between rat groups' portal inflammation rates were found and the moderate and severe ballooning were observed in 30% fructose rats (p<0.05). Conclusions: As a result, dietary fructose from added sugar as high fructose corn syrup may causes major metabolic disorders.
Evaluation of New Baskent University Preservation Solution for Kidney Graft During Cold Ischemia: Preliminary Experimental Animal Study
(2019) Haberal, Mehmet; Kirnap, Mahir; Erdem, S.Remzi; Ozdemir, B.Handan; Lux, K. Michael; Bacanli, Didem; 31145052
Objectives: Organ damage due to long cold ischemia time remains a hurdle in transplantation. In this preliminary animal study, we compared the new Baskent University Preservation Solution (BUPS) with the University of Wisconsin (UW) and histidine-tryptophan-ketoglutarate (HTK) solutions. Materials and Methods: BUPS composition included electrolytes, raffinose, mannitol, N-acetylcysteine, taurine, adenosine, and ascorbic acid. In experiment 1, kidneys from 50 male Sprague-Dawley rats were placed into BUPS, HTK, or UW solution to assess cold ischemia injury, with biopsies taken at different time points for pathologic evaluation. In experiment 2, to investigate ischemia-reperfusion injury, 5 rats were renal transplant donors to 10 rats and 6 pigs were used as transplant donors-recipients among each other. Results: In experiment 1, no significant cellular injury was shown at up to 3 hours of perfusion with any solution. At 6- to 48-hour perfusion, tubular injury was shown, with lowest injury in BUPS and HTK versus UW and control groups (P < .01). The BUPS group showed more moderate degree of tubular apoptosis and cytoskeletal rearrangement than the HTK and UW groups at 12-, 24-, and 48-hour perfusion (P < .01). In experiment 2, after ischemia-reperfusion injury, no significant differences were found between HTK and BUPS groups regarding tubular damage. Although no significant differences were shown regarding tubular cytoskeletal rearrangment and apoptosis in pig reperfusion group with BUPS versus HTK, significant differences were shown with these solutions in other groups. Conclusions: Tubular damage during ischemia-reperfusion injury (cytoskeletal disruption, increased apoptosis) were lower with BUPS. BUPS can be a costeffective perfusion solution in transplantation.
Evaluation of new Baskent university preservation solution for kidney graft during cold ischemia: Preliminary experimental animal study.
(2019) Haberal, Mehmet; Kirnap, Mahir; Erdem, S.Remzi; Ozdemir, B.Handan; Lux, K.Michael; Bacanli, Didem
EVALUATION OF NEW BASKENT UNIVERSITY PRESERVATION SOLUTION FOR LIVER, KIDNEY AND INTESTINE GRAFT DURING COLD ISCHEMIA: PRELIMINARY EXPERIMENTAL ANIMAL STUDY
(2019) Haberal, Mehmet; Kirnap, Mahir; Erdem, Remzi; Ozdemir, B. Handan; Lux, K. Michael; Bacanli, Didem; AAH-9198-2019
Preliminary Experimental Model for "Ileobladder" and Renal Transplant in Rats and Pigs
(2017) Haberal, Mehmet; Kirnap, Mahir; Gokce, Oruc Numan; Bacanli, Didem; Ersoy, Zeynep; Bayzakov, Mirbek; Torgay, Adnan; Ozdemir, Handan; 0000-0002-3462-7632; 0000-0002-6829-3300; 0000-0003-0767-1088; 0000-0002-6829-3300; 0000-0002-7528-3557; AAJ-8097-2021; AAH-9198-2019; AAJ-5221-2021; AAF-3066-2021; AAJ-5221-2021; X-8540-2019
Prevention of Vascular Stricture By Using An Expanded Polytetrafluoroethylene (Eptfe) Graft in A Porcine Model: Preliminary Study
(2016) Haberal, Mehmet; Ozdemir, Handan; Ozcay, Necdet; Bacanli, Didem; Ersoy, Zeynep; Torgay, Adnan; https://orcid.org/0000-0002-3462-7632; https://orcid.org/0000-0003-0767-1088; https://orcid.org/0000-0002-6829-3300; AAJ-8097-2021; AAF-3066-2021; AAJ-5221-2021
Radiological and Histological Evaluation of the Effects of Cortical Perforations on Bone Healing in Mandibular Onlay Graft Procedures
(2016) Dayangac, Emre; Araz, Kenan; Oguz, Yener; Bacanli, Didem; Caylak, Berrin; Uckan, Sina; 24889104
BackgroundPerforations of the cortical bone may be an advantage for the success of the autogenous bone graft procedure, but whether this perforation has a positive effect on the bone remains controversial. PurposeThis study evaluates the effects of cortical perforation of the autogenous bone block graft radiologically and histologically. Materials and MethodsSeven adult pigs were used for this study. On the experimental side, cortical perforation at the host site was prepared, while no perforation was done on the control side. The specimens were evaluated, and the Wilcoxon signed-rank test was used for statistical analysis. ResultsIn the radiological evaluation, the Wilcoxon signed-rank test indicated no significant differences in densities among the grafts (p=.23) with a mean of 4.290.951 for the unperforated graft side and 3.57 +/- 0.976 for the decorticated graft side. In histological evaluation, there was a significant difference in the thickness of the grafts between the groups (experimental group 3.71 +/- 1.286, control group: 4.71 +/- 0.488; p=.033). However, when the remodeling and osteoblastic activity in the grafts were measured, no significant differences were observed between the groups (p=1 and p=.133, respectively). ConclusionIn augmentation with mandibular onlay bone grafts, cortical perforations in the recipient site make no distinct contribution to bone healing within 12 weeks.
Technique of Ileobladder and Kidney Transplant in Rats and Pigs
(2018) Haberal, Mehmet; Kirnap, Mahir; Gokce, Oruc N.; Bacanli, Didem; Ersoy, Zeynep; Bayzakov, Mirbek; Torgay, Adnan; Ozdemir, Handan; Erdem, Remzi; 0000-0003-0767-1088; 0000-0002-9678-7818; 0000-0002-7528-3557; 0000-0002-6829-3300; 0000-0002-3462-7632; 0000-0002-7537-2170; 29409436; AAF-3066-2021; AAH-9198-2019; AAQ-8259-2021; X-8540-2019; AAJ-5221-2021; AAJ-8097-2021
Objectives: Kidney transplant is the best choice for treatment of patients with advanced chronic renal disease. However, small, poorly compliant, and unstable bladders can result in major problems for patients. Here, we aimed to develop and evaluate a new ileobladder model. Materials and Methods: Fifteen rats (250-300 g) and 5 pigs (similar to 100 kg) were cared for according to institutional and published guidelines. After general anesthesia, laparotomy was done through midline incision. Ileal loops were prepared for ileobladder. After cystectomy (0.5 cm above the trigone in rats, 1 cm above the trigone in pigs), anastomoses were done between antimesenteric sides of ileal loops and bladder remnant with 6/0 Prolene suture. Three other pigs received simultaneous renal transplant. Results: One rat died on day 1 postsurgery from multiorgan hemorrhage. Two rats survived for 5 days, 3 rats for 7 days, and 3 rats for 11 days; 6 rats were killed for pathologic evaluation after 3 months. One pig survived for 22 days and 1 for 9 days. Of the 3 pigs that received a simultaneous renal transplant, 2 pigs were alive and doing well 80 and 72 days after surgery with normal urinary discharge (1 pig was killed for pathologic evaluation after 3 days). When ileobladder was opened, complete recovery of the anastomosis line was observed. Pathologic examination of the anastomosis sites reported a normal healing process with moderate inflammation and the muscular wall of the intestine showed hypertrophia that nearly reached the size of the bladder muscularis propria. Conclusions: Although we had some complications because no draining procedure was used, in terms of technique, our new ileobladder model is promising for providing functional bladder volume. A larger scale series in the clinical setting is planned. This technique can be useful for small bladders and bladder physiology disorders.