Browsing by Author "Ayva, Ebru Sebnem"

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A Case of Common Variable Immunodeficiency with CREBP Gene Mutation without Rubinstein Taybi Syndrome Features
(2022) Musabak, Ugur; Ceylaner, Serdar; Erdogan, Tuba; Ayva, Ebru Sebnem; 0000-0003-1511-7634; 35833092; AAU-1810-2020
Hypogammaglobulinemias, based on inborn errors of immunity, are primary immunodeficiencies (PIDs) that can also be diagnosed for the first time in adulthood. Common variable immunodeficiency (CVID) is a multifactorial disease often symptomatic due to antibody deficiency. In addition, some PIDs are classified into the category of immunodeficiencies with syndromic features due to their accompanying clinical findings unrelated to immunity. In this article, a patient with CVID who was diagnosed in adulthood and who was revealed to have a mutation specific to Rubinstein-Taybi syndrome and clinical features reminiscent of this syndrome only after molecular tests was presented.
Clinical Use of Tumor Markers for the Detection and Prognosis of Bladder Carcinoma: A Comparison of CD44, Cytokeratin 20 and Survivin
(2016) Yikilmaz, Taha Numan; Dirim, Ayhan; Ayva, Ebru Sebnem; Ozdemir, Handan; Ozkardes, Hakan; https://orcid.org/0000-0003-2898-485X; https://orcid.org/0000-0002-2280-8778; https://orcid.org/0000-0002-7528-3557; https://orcid.org/0000-0002-7277-449X; 27351322; AAJ-5689-2021; AAK-1967-2021; X-8540-2019; AAH-1052-2020
Purpose: To investigate the role of CD44, cytokeratin 20 (CK20) and survivin for the detection and prognosis of patients with urothelial carcinoma of the bladder. Materials and Methods: The study included 82 patients who underwent transurethral resection of bladder tumors between 2009 and 2014. The patient and tumor characteristics with relevance to age, tumor size and focality, grade and stage, recurrence and progression were noted. Patients with carcinoma in situ, those who had at more than 3 sites of lesions and greater than 3 cm tumors were excluded. All cases were ex-smokers. All histological samples stained with hematoxylin and eosin were re-evaluated according to the 2004 World Health Organization/International Society of Urological Pathology (WHO/ISUP) classification system and immunohistochemically stained for CD44, CK20 and survivin. Results: The study group comprised 57 (69.5%) males and 25 (30.5%) females with a mean age of 60 years (range, 26-87 years). All were newly-diagnosed patients with bladder tumors. Immunohistochemical evaluation revealed that there was a statistically significant correlation between the grade and stage of the tumor with CK20 and survivin positivity (P < .05). As the grade and stage increased CD44 immunoreactivity significantly decreased (P = .002, P = .0001, respectively). However, relationship of protein expressions with recurrence and progression remained insignificant (P > .05). Conclusion: In cases of bladder urothelial carcinoma positivity for CD44, CK20, and survivin has significant relation with the tumor grade and stage while no significant relationship was determined in terms of recurrence and progression
The effect of extracorporeal shock wave lithotripsy on distribution of interstitial cells of Cajal in rabbit renal pelvis and proximal ureter
(2015) Boybeyi, Ozlem; Fedakar Senyucel, Mine; Ayva, Ebru Sebnem; Soyer, Tutku; Aslan, Mustafa Kemal; Basar, Mehmet Murad; Cakmak, Ahmet Murat; 25790556
Background/aim: An experimental study was performed to evaluate the effect of extracorporeal shock wave lithotripsy (ESWL) on the distribution of interstitial cells of Cajal (ICC) in rabbit renal pelvis and proximal ureter. Materials and methods: Six New Zealand rabbits were included. Right kidneys were exposed to a total of 3000 shock waves (14 kV) by using an electrohydraulic-type ESWL device. Right sides were allocated as the ESWL group (EG, n = 6) and left sides as the control group (CG, n = 6). Tissues were harvested on day 7. Tissues were examined histopathologically for the presence of edema, inflammation, congestion, hemorrhage, fibrosis, and vascularization. Mast cell tryptase and CD117 (c-kit) staining was performed for ICC distribution. Results: Although increased tissue edema in renal pelvises and increased inflammation in ureters were observed in EG, no statistical difference was detected between groups (P > 0.05). In CG, positive CD117 staining was detected in 2 renal pelvises and ureters. None of the EG samples showed CD117 staining and no statistical difference was detected between groups (P > 0.05). Conclusion: Rabbit does not appear to be a good model for investigating ICCs. ESWL may cause histopathological alterations in the renal pelvis and ureter. Since it has not been statistically proven, reduced contractility of the ureter after ESWL may not be attributed to altered distribution of ICCs in the renal pelvis and ureter.
Extraocular Sebaceous Carcinoma in Muir-Torre Syndrome
(2022) Albayati, Abbas; Ozkan, Burak; Ayva, Ebru Sebnem; Uysal, Ahmet Cagri; Ertas, Nilgun Markal; 0000-0003-2806-3006; 36092191; AAC-3344-2021
Impact of Vitamin D3 on Cytoskeletal Reorganization and Regulation of Adherens Junctions of Tubule Cells: Epithelial to Mesenchymal Transition and Development of Interstitial Fibrosis in Renal Allografts
(2018) Ozdemir, B. Handan; Ozdemir, Fatma Nurhan; Ayva, Ebru Sebnem; Akcay, Eda Yilmaz; Soy, Ebru H. Ayvazoglu; Ozdemir, Gokce; Polat, Aysegul Yucel; Haberal, Mehmet; 0000-0002-7528-3557; 0000-0002-5682-0943; 0000-0002-2280-8778; 0000-0002-0993-9917; 0000-0003-2545-0078; 0000-0002-3462-7632; X-8540-2019; AAK-1697-2021; AAK-1967-2021; AAC-5566-2019; AAL-4282-2020; AAJ-8097-2021
Lung Biopsy Findings of Patients Who Have Undergone a Liver and Kidney Transplant
(2016) Ozgun, Gonca; Tepeoglu, Merih; Ozdemir, Binnaz Handan; Akcay, Eda Yilmaz; Ayva, Ebru Sebnem; Deniz, Emine Ebru; Haberal, Mehmet; 0000-0002-9894-8005; 0000-0002-7528-3557; 0000-0001-6831-9585; 0000-0002-2280-8778; 0000-0002-3462-7632; 27805533; AAK-5222-2021; X-8540-2019; AAK-1960-2021; AAK-1967-2021; AAJ-8097-2021
Objectives: Our objective was to analyze the incidence of pulmonary disorders in solid-organ transplant recipients and report on outcomes in these patients. Materials and Methods: Seventy liver and kidney transplant patients, who underwent lung biopsy because of pulmonary symptoms between January 2000 and December 2015, were enrolled in the study. We examined and evaluated histopathologic findings of these patients based on clinical data recorded in patients' files. Results: Patients' mean age was 44.5 +/- 16.4 years. Of 70 patients, 25 underwent liver transplant and 45 patients underwent kidney transplant. Forty-six patients received organs from living donors and 24 from deceased donors. Biopsy results of all patients included nonspecific findings (28), organized pneumonia (2), tuberculosis (6), fungal infections (11), tumors (5), amyloidosis (1), diffuse alveolar damage (4), mixed bacterial infection (1), and bronchopneumonia (12). Forty-two patients (60%) died within 54.1 +/- 53.3 months after transplant and 24.6 +/- 41.9 months after lung biopsy. Autopsies were performed on 14 patients. The causes of fatal lung diseases included fungal infections (8), malignant tumors (4), amyloidosis (1), diffuse alveolar damage (4), and mixed bacterial infection (1). Aspergillosis was the most frequently implicated lung infection, occurring in 54.5% of patients with fungal infections. Conclusions: Pulmonary diseases remain an important cause of morbidity and mortality in solid-organ transplant recipients. Fungal infection, especially aspergillosis, was the leading cause of early death in these patients.
Metachronous Occurrence of Granular Cell Tumor in Breast Skin and Scalp: Diagnostic Challenging Differentiating Benign from Malignant and a Literature Review
(2016) Akkaya, Hampar; Toru, Havva Serap; Ayva, Ebru Sebnem; Karabulut, Zulfikar; Durusoy, Cicek; 26881167
Granular cell tumor (GCT) is a Schwann cell related benign neoplasm of soft tissue. GCT is an uncommon entity that occurs in a wide variety of body sites, but it is generally presented in the skin, oral cavity, superficial soft tissue, and respiratory and digestive tracts. Most of the GCTs are benign but clinically and radiologically these may mimic malignancy. Histopathological diagnosis is gold standard for establishing the true nature of the lesion. GCT is most commonly solitary but in about 10% of cases can be multifocal, usually involving various skin and soft tissue sites versus involving various internal sites. Therefore, these can involve skin and soft tissue or submucosa and viscera. GCT is usually benign; however, local recurrence is common due to incomplete removal. Malignant cases are rarely reported in 1-2% of cases. In this study, we report clinical and histopathological findings of a 36-year-old woman with metachronous GCT in breast and scalp. The clinical features raise the question of whether these are metachronous benign GCTs or whether this is establishment of malignant behavior. The aim of this report is to present the histopathological and clinical features of GCT and the diagnostic challenge of differentiating benign from malignant GCT.
MiR-25 and KLF4 relationship has early prognostic significance in the development of cervical cancer
(2021) Polat, Aysegul Yucel; Ayva, Ebru Sebnem; Gurdal, Hakan; Ozdemir, Binnaz Handan; Dedeoglu, Bala Gur; 0000-0002-7528-3557; 33862560; X-8540-2019
Cervical squamous cell carcinoma (SCC) is one of the common cancer types among women. MicroRNAs (miRNAs) are small non-coding RNAs that play an important role in the formation and development of many cancer types by regulating expression of their targets. While many studies have investigated the relationship between miRNAs and cervical cancer, no robust miRNA biomarkers have been defined yet for diagnosis of cervical lesions. In this study, we performed a statistical meta-analysis to identify miRNAs and a class compassion analysis to evaluate mRNAs with the power to discriminate between normal, intraepithelial lesions and invasive cancer samples. Differentially expressed (DE) mRNAs were compared with the targets of meta-miRNAs. After bioinfomatics analysis and qRT-PCR validations with cytology samples and FFPE tissues, we defined miR-25 and its target KLF4 (Kruppel-like factor 4) as candidate biomarkers for in vitro studies. Our results showed that miR-25 expression was significantly higher in precancerous lesions and invasive carcinoma while presenting consistent expression patterns in both cytological and FFPE tissue samples. In line with this, its direct target KLF4 expression decreased in precancerous lesions in cytological samples and also in the invasive cancer group in FFPE tissues. Furthermore, in vitro studies showed that mir-25 inhibition decreased proliferation and motility of HeLa cells and promoted an increase in the protein level of KLF4. We conclude that inhibition of miR-25 may upregulate KLF4 expression and regulate cell proliferation and motility in cervical cancer.
p16(INK4a), and p14(ARF) Expressions in Carcinogenesis of Squamous Cell Carcinoma of the Lip
(2022) Akatli, Ayse Nur; Ayva, Ebru Sebnem; Bozdogan, Onder
The goal of this study was to clarify the role of p16(INK4a), p14(ARF), and p53 protein expressions in carcinogenesis in squamous cell carcinomas of the lip. The expressions of the p53, p16(INK4a), and p14(ARF) proteins were examined in 46 formaline-fixed paraffin embedded tissue specimens, which included 19 cases of squamous cell carcinoma of the lip, 14 cases of actinic cheilitis, and 13 cases of normal mucosa. Immunoreactivity in the peritumoral epithelium adjacent to squamous cell carcinomas was also evaluated. p16(INK4a) expression was increased in actinic cheilitis in comparison with normal mucosa (p=0.001). p14(ARF) expression progressively increased from normal mucosa to actinic cheilitis (p=0.001) and was observed to decrease significantly during the process of transition from actinic cheilitis to carcinoma (p=0.003). p53 values progressively increased from normal mucosa to actinic cheilitis (p=0.001) and carcinoma (p=0.008). A significant positive correlation was found between p14(ARF) and p53 in the peritumoral epithelium adjacent to carcinomas. Our findings indicated that p16(INK4a) and p14(ARF) immunohistochemistry does not determine whether or not actinic cheilitis has the potential to develop carcinoma. The p14(ARF) /p53 pathway is activated in the peritumoral epithelium adjacent to carcinoma; however, this activation would not be adequate to prevent carcinogenesis.
Rearrangement of the Endothelial Cell Cytoskeleton in Glomerular and Peritubular Capillaries after Antibody-Mediated Rejection: Significance in the Development of Transplant Glomerulopathy and Interstitial Fibrosis
(2018) Ozdemir, B. Handan; Ozdemir, Fatma Nurhan; Ayva, Ebru Sebnem; Akcay, Eda Yilmaz; Soy, Ebru H. Ayvazoglu; Haberal, Mehmet; 0000-0002-7528-3557; 0000-0002-5682-0943; 0000-0002-2280-8778; 0000-0001-6831-9585; 0000-0002-0993-9917; 0000-0002-3462-7632; X-8540-2019; AAK-1697-2021; AAK-1960-2021; AAK-1967-2021; AAJ-8097-2021
A Single-Center Retrospective Clinicopathologic Study of Endomyocardial Biopsies After Heart Transplant at Baskent University Hospital in Ankara, 1993-2014
(2015) Terzi, Ayen; Sezgin, Atilla; Tunca, Zeynep; Deniz, Ebru; Ayva, Ebru Sebnem; Reyhan, Nihan Haberal; Mulderrisoglu, Haldun; Ozdemir, Binnaz Handan; 0000-0002-2280-8778; 0000-0002-7528-3557; 0000-0002-1225-1320; 0000-0001-9852-9911; 25894188; AAK-1967-2021; X-8540-2019; F-7546-2013; AAK-4587-2021
Objectives: The purpose of this study was to investigate the frequency and prognostic importance of acute cellular rejection after heart transplant. Materials and Methods: All 84 heart transplant patients at our center from January 1993 to January 2014, including all 576 endomyocardial biopsies, were evaluated with retrospective review of clinical records and endomyocardial biopsies. Routine and clinically indicated endomyocardial biopsies after heart transplant were graded for acute cellular rejection (2005 International Society for Heart and Lung Transplantation Working Formulation). Survival analysis was performed using Kaplan-Meier method. Results: There were 61 male (73%) and 23 female recipients. Median age at heart transplant was 29 years (range, 1-62 y). Posttransplant early mortality rate was 17.9% (15 patients). In the other 69 patients, 23 patients died and 46 patients (66.7%) were alive at mean 69.3 +/- 7.2 months after heart transplant. Mean follow-up was 35.4 +/- 29.8 months (range, 0.07-117.5 mo). Mean 8.4 +/- 4.2 endomyocardial biopsies (range, 1-19 biopsies) were performed per patient. Median first biopsy time was 7 days (range, 1-78 d). The frequency of posttransplant acute cellular rejection was 63.8% (44 of 69 patients) by histopathology; 86% patients experienced the first episode of acute cellular rejection within 6 months after transplant. There were 18 patients with acute cellular rejection >= grade 2R on >= 1 endomyocardial biopsy in 44 patients with acute cellular rejection. No significant difference was observed between survival rates of patients with grade 1R or >= grade 2R acute cellular rejection, or between survival rates of patients with or without diagnosis of any grade of acute cellular rejection. Acute cellular rejection was not related to any prognostic risk factor. Conclusions: Acute cellular rejection had no negative effect on heart recipient long-term survival, but it was a frequent complication after heart transplant, especially within the first 6 months.
Subcutaneous and Muscular Metastasis of Pancreatic Adenocarcinoma
(2017) Haberal, Kemal Murat; Donmez, Fuldem Yildirim; Ayva, Ebru Sebnem; Coskun, Mehmet; 0000-0002-8211-4065; 0000-0003-4502-106X; 0000-0002-2280-8778; R-9398-2019; AAE-5528-2021; AAK-1967-2021
Soft tissue metastasis from pancreatic cancer is a very rare entity. We reported a case presenting with multiorgan and soft tissue metastasis on computed tomography. Computed tomography scan showed a hypovascular mass in the pancreatic body and tail. Computed tomography guided biopsy from muscle metastases diagnosed an undifferentiated tumor. Based on clinical, radiological and pathological correlation, the diagnosis of pancreatic adenocarcinoma was established. There are few reported cases of skeletal muscle metastasis from pancreatic cancer. Immunohistochemical staining and imaging findings should be used together to determine the correct diagnosis.