Browsing by Author "Aydin, Leyla"

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Adipose-Derived Stem Cells Enhance Axonal Regeneration through Cross-Facial Nerve Grafting in a Rat Model of Facial Paralysis
(2016) Abbas, Ozan L.; Borman, Huseyin; Uysal, Cagri A.; Gonen, Zeynep B.; Aydin, Leyla; Helvacioglu, Fatma; Ilhan, Sebnem; Yazici, Ayse C.; https://orcid.org/0000-0001-6236-0050; https://orcid.org/0000-0002-6026-0045; https://orcid.org/0000-0002-3132-242X; 27465163; AAJ-2949-2021; AAH-8887-2021; AAS-6810-2021
Background: Cross-face nerve grafting combined with functional muscle transplantation has become the standard in reconstructing an emotionally controlled smile in complete irreversible facial palsy. However, the efficacy of this procedure depends on the ability of regenerating axons to breach two nerve coaptations and reinnervate endplates in denervated muscle. The current study tested the hypothesis that adipose-derived stem cells would enhance axonal regeneration through a cross-facial nerve graft and thereby enhance recovery of the facial nerve function. Methods: Twelve rats underwent transection of the right facial nerve, and cross-facial nerve grafting using the sciatic nerve as an interpositional graft, with coaptations to the ipsilateral and contralateral buccal branches, was carried out. Rats were divided equally into two groups: a grafted but nontreated control group and a grafted and adipose-derived stem cell-treated group. Three months after surgery, biometric and electrophysiologic assessments of vibrissae movements were performed. Histologically, the spectra of fiber density, myelin sheath thickness, fiber diameter, and g ratio of the nerve were analyzed. Immunohistochemical staining was performed for the evaluation of acetylcholine in the neuromuscular junctions. Results: The data from the biometric and electrophysiologic analysis of vibrissae movements, immunohistochemical analysis, and histologic assessment of the nerve showed that adipose-derived stem cells significantly enhanced axonal regeneration through the graft. Conclusion: These observations suggest that adipose-derived stem cells could be a clinically translatable route toward new methods to enhance recovery after cross-facial nerve grafting.
An Alternative Low-Cost Solution for Tracking Laboratory Animals
(2020) Kiziltan, Erhan; Aydin, Leyla; 0000-0001-6029-3835; AAD-6090-2021
Objectives: Monitoring animal behavior under various conditions can provide important information on their neuropsychological status, including learning, memory, and cognitive activity. Several commercial tracking systems are available, but they may be too expensive for low-budget projects. This study developed an alternative solution for automated animal tracking in behavioral experiments. Methods: The proposed system was designed to analyze a set of images sampled from a recorded video file in chronological order. The instantaneous location of the animal in each image frame was defined automatically, using a feature-extraction algorithm. Distances traveled were calculated using the coordinates of the successive instantaneous locations. The algorithm was tested using two arenas: the Morris water maze and open field test. The calculated measures were compared with those obtained manually. The internal consistency of the dataset was checked using Cronbach's alpha. The accuracy of the results was evaluated using the paired samples t-test and Pearson correlation, with the level of statistical significance set at p<0.01. Results: A statistical comparison of the distances traveled, which were derived from the coordinates of successive locations, did not differ significantly between the manual and automatic methods (r=0.954 and p=0.792 for the Morris water maze; r=0.996 and p=0.024 for the open field test). Conclusions: These results suggest that the algorithm is reliable and valid for estimating coordinates and may serve as a high-resolution tool for animal behavior experiments. We intend to make this software freely available to interested readers and to open feedback channels for further development.
Anticonvulsant Efficacy of Melatonin in an Experimental Model of Hyperthermic Febrile Seizures
(2015) Aydin, Leyla; Gundogan, Nimet Unay; Yazici, Canan; 0000-0002-3132-242X; 0000-0001-7784-9641; 26600370; AAS-6810-2021; ABC-5392-2020; AAK-4338-2021
Introduction: The anticonvulsant effects of melatonin (MT) have been demonstrated in several different experimental seizure models. Thus, the present study aimed to determine the anticonvulsant efficacy of MT, the optimum time for its administration prior to the induction of a seizure, and its effective dose in a rat model of hyperthermic febrile seizures (FSs). Methods: The present study included 72 male Sprague-Dawley rat pups divided into eight groups. The seizures were induced by keeping the rats in 45 degrees C water and the experiments were performed in two steps. In the first step, the control group was given a vehicle injection and the study groups were given a MT injection (150 mg/kg, intraperitoneal [i.p.]) at either 5, 10, or 15 min prior to the induction of the seizure to determine the anticonvulsant effects of MT and its optimum time of administration. In the second step, a vehicle injection and three different doses of MT (80, 100, and 150 mg/kg, i.p.) were administered 15 min prior to the induction of the seizure to determine the dose at which anticonvulsant effects could be achieved. The anticonvulsant effects were assessed based on the latency of the FSs. Results: In the first-step experiments, the FS latency of the control group was 143.4 +/- 15.3 s and the latencies of the groups given melatonin at either 5, 10, or 15 min prior to the seizure were 174.2 +/- 28.9, 177.4 +/- 21.0, and 193.7 +/- 17.6 s, respectively. Compared with the control group, the latencies for each of the study groups were significantly longer (p < 0.001), with the longest latency observed in the group given melatonin 15 min before the seizure. In the second-step experiments, the FS latencies of the groups that were given 80,100, and 150 mg/kg of MT 15 min before the seizure were 238.7 +/- 4.0, 240.0 +/- 0.0, and 193.7 +/- 17.6 s, respectively. These latencies were significantly longer than those of the control group (172.3 +/- 30.3 s, p < 0.001). Conclusion: The present study demonstrated that MT exerts anticonvulsant effects in a rat model of hyperthermic FSs and achieved its optimum efficacy at a dose of 80 mg/kg when administered 15 min prior to the induction of a seizure. (C) 2015 Elsevier B.V. All rights reserved.
Cognitive Deterioration Following Strength Training in Adolescents
(2019) Yargic, MP; Aydin, Leyla; Erdagi, Kenan; Kiziltan, Erhan; https://orcid.org/0000-0001-6029-3835; AAD-6090-2021
Determining the Opinions of Baskent University Health Sciences Faculty Students on the Course and Education of Physiology
(2017) Aydin, Leyla; Sencelikel, Tugce; ABC-5392-2020
Effect of Melatonin on Cytokine Levels in A Hyperthermia-Induced Febrile Seizure Model
(2017) Aydin, Leyla; Yurtcu, Erkan; Korkmaz, Yesim; Sezer, Taner; Ogus, Ersin; 0000-0003-4930-8164; 0000-0002-2278-1827; 0000-0002-9877-421X; 29208169; ABC-5392-2020; AAA-2998-2021; AAJ-5931-2021; AAJ-1058-2021
Higher serum cytokine levels have been reported in children admitted with febrile seizures and in some experimental models. However, other studies have shown that cytokine levels are influenced by melatonin. In this study, we investigated serum cytokine levels in a hyperthermia-induced febrile rat seizure model and the effect of melatonin. A total of 28 male Sprague-Dawley rats were divided into four groups: the control (C) group, healthy melatonin (MT) group, and hyperthermia-induced febrile seizure groups with (HIFS-MT) and without (HIFS) administration of melatonin. Melatonin (80 mg/kg) was given intraperitoneally 15 min before the seizure. HIFS was induced by placing the rats in 45 degrees C water. The rats were sacrificed under anesthesia after the seizure. Blood samples were drawn by transcardiac puncture to measure serum cytokine and melatonin levels. Serum interleukin (IL)-1 beta, IL-6, IL-10, and tumor necrosis factor (TNF)-alpha levels were lower in the HIFS group than those in the C group (p = 0.005, p = 0.200, p = 0.011, and p = 0.016, respectively). All serum cytokine levels of rats in the MT and HIFS-MT groups were similar to those in the C group. This experimental rat model demonstrated that serum cytokine levels decrease with HIFS and that administering melatonin maintains serum cytokine levels. These results suggest that cytokines may play role in the anticonvulsive activity of melatonin in rats with febrile seizures.
The Impacts of Central Fatigue on the Polyphasic Nature of Tapping Performance
(2018) Kiziltan, Erhan; Aydin, Leyla; Ogus, Ersin; Azizagaoglu, Bahadir; Buyukkaraman, Arda; Doga, Selen; Ertuk, Gizem; Kus, Cansel; 0000-0002-9877-421X; 0000-0001-6029-3835; AAJ-1058-2021; ABC-5392-2020; AAD-6090-2021
Objective: As a non-specific symptom muscle fatigue mostly accompanies neuromuscular diseases and also occurs frequently in healthy individuals. Fatigue phenomenon is considered to be multidimensional symptom. There have been still discussions on the origin whether it depends primarily on the intrinsic properties of muscle itself (peripheral mechanisms) or the nervous system that controls muscle (central mechanisms). This study aimed to investigate the effects of central fatigue on the performance of maximal voluntary repetitive movement and discusses the specificity of finger tapping task test as a simple diagnostic tool for fatigue. Methods: For this purpose, 27 healthy, male, right-handed volunteer performed the 20-s of finger tapping task test for four times. The one was for control and the other three were performed right after induction of three different central fatigue models. Temporal behavior of tapping performances were evaluated based on inter-tap intervals and the statistical comparison were made by regression analysis. Results: The results showed that the partial evaluation of the task in time domain instead of complete test period yielded with statistically significant differences between control and fatigue models (p<0.001) and even in between the fatigue models. Conclusion: Approximately the first 5-s of a finger tapping task consists of both motor learning processes and dynamics of energy consumption from anaerobic sources. However, it reflects dominantly the central components of fatigue. We may conclude that the temporal behavior of tapping performance following the induction of specific fatigue model may help making further discrimination for the origin of fatigue.
Internal Motivation Modulates Voluntary Repetitive Movements: "Ha gayret" Energy
(2017) Kiziltan, Erhan; Aydin, Leyla; Zengin, H. Yagmur; 0000-0001-6029-3835; 0000-0002-9855-2449; AAD-6090-2021; ABC-5392-2020; ABA-3224-2021
Is Central Origin of Muscle Fatigue Distinguished Solely in Finger Tapping Performance?
(2017) Aydin, Leyla; Buyukkaraman, Arda; Kiziltan, Erhan; Dogan, Selen; Ogus, Ersin; Erturk, Gizem; Azizagaoglu, Bahadir; Kus, Cansel; 0000-0001-6029-3835; 0000-0002-9877-421X; ABC-5392-2020; AAD-6090-2021; AAJ-1058-2021
As a non-specific symptom muscle fatigue mostly accompanies neuromuscular diseases and also occurs frequently in healthy individuals. Fatigue phenomenon is considered to be multidimensional symptom. There have been still discussions on the origin whether it depends primarily on the intrinsic properties of muscle itself (peripheral) or the nervous system that controls muscle (central). This study aimed to investigate the effects of central fatigue on finger tapping performance and discusses the specificity of finger tapping task test as a simple diagnostic tool for fatigue. For this purpose, 27 healthy, male, right-handed volunteer performed four 20-s of finger tapping task tests. The one was for control and the other three were followed by three different central fatigue models. Temporal behavior of tapping performances were evaluated based on inter-tap intervals and the statistical comparison were made by regression analysis. The results showed that the partial evaluation of the task instead of complete test period yielded with statistically significant differences between control and fatigue models (p<0.001) and even in between fatigue models. Approximately the first 5-s of a finger tapping task consists of motor learning processes and dynamics of energy consumption from anaerobic sources therefore, reflects the central components of fatigue, dominantly. We may conclude that the temporal behavior of tapping performance following the induction of specific fatigue model may help making further discrimination for the origin of fatigue.
Laboratory Animal Tracking in Behavioral Experiments: An Alternative Low Cost Solution
(2016) Kiziltan, Erhan; Aydin, Leyla; 0000-0001-6029-3835; AAD-6090-2021; ABC-5392-2020
Polyphasic Temporal Behavior of Finger-Tapping Performance: A Measure of Motor Skills and Fatigue
(2016) Aydin, Leyla; Kiziltan, Erhan; Gundogan, Nimet Unay; https://orcid.org/0000-0001-6029-3835; https://orcid.org/0000-0001-7784-9641; 25988674; ABC-5392-2020; AAD-6090-2021; AAK-4338-2021
Successive voluntary motor movement involves a number of physiological mechanisms and may reflect motor skill development and neuromuscular fatigue. In this study, the temporal behavior of finger tapping was investigated in relation to motor skills and fatigue by using a long-term computer-based test. The finger-tapping performances of 29 healthy male volunteers were analyzed using linear and nonlinear regression models established for inter-tapping interval. The results suggest that finger-tapping performance exhibits a polyphasic nature, and has several characteristic time points, which may be directly related to muscle dynamics and energy consumption. In conclusion, we believe that future studies evaluating the polyphasic nature of the maximal voluntary movement will lead to the definition of objective scales that can be used in the follow up of some neuromuscular diseases, as well as, the determination of motor skills, individual ability, and peripheral fatigue through the use of a low cost, easy-to-use computer-based finger-tapping test.
Relationship Between Academic Successes of Physiology and Pathology Courses
(2017) Aydin, Leyla; Ogus, Ersin; 0000-0002-9877-421X; ABC-5392-2020; AAJ-1058-2021
The Role of Finger Tapping Task in the Assessment of Central Fatigue
(2016) Aydin, Leyla; Kiziltan, Erhan; Ogus, Ersin; Azizagaoglu, Bahadir; Buyukkaraman, Arda; Dogan, Selen; Erturk, Gizem; Kus, Cansel; 0000-0002-9877-421X; 0000-0001-6029-3835; ABC-5392-2020; AAJ-1058-2021; AAD-6090-2021
Temporal Analysis of Finger-Tapping Test in Individuals over 65 Years of Age: Relation of Motor Function and Energy Consumption
(2015) Aydin, Leyla; Aydin, Aydin; Bulbul, Baris; Ozkalkan, Beliz; Ozaydin, Cansu; Inaltekin, Ezgi; ABC-5392-2020
The Use of Computer Based Test Battery for The Assessment of Cognitive Functions in Elite-Level Strength Training
(2019) Yargic, Melda Pelin; Erdagi, Kenan; Aydin, Leyla; Kiziltan, Erhan; https://orcid.org/0000-0001-6029-3835; AAD-6090-2021
The aim of this study was to propose a standard test battery consisting of necessary tools for measuring and comparing the various aspects of cognitive outcomes. The battery was used to determine whether adolescent women performing regular elite-level strength training differed from their sedentary peers in terms of cognition, and also to determine how a single session of strength training affects cognition in highly trained adolescents. Motor functions, ability of sustaining attention and executive functions of 25 elite female weightlifters and 22 sedentary females were evaluated through finger tapping performance, visual reaction time (VRT) and recognition visual reaction time (R-VRT) data. Weightlifters were tested before and after a training session, sedentary controls were tested only during resting. There was a significant increase in mean complex R-VRT of weightlifters after training (p<0.01). In R-VRT tests, rate of false answers increased significantly after training (p<0.05). Mean VRT of weightlifters (during rest) and sedentary peers were not different in any of the tests (p>0.05). Total number of taps and mean inter-tap intervals did not show any difference in the weightlifter group before and after training, also between weightlifters and sedentary controls (p>0.05). Elite level strength training does not improve cognition in adolescence. Adolescent weightlifters' executive functions are deteriorated following a single training session however, this effect is temporary.