Browsing by Author "Aydin, Arif"

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    The Protective Effect of Lycopene on Kidney Against Experimentally Induced Unilateral Ureteral Obstruction
    (2016) Atilgan, Hasan Ikbal; Aydin, Arif; Sadic, Murat; Korkmaz, Meliha; Karakan, Tolga; Ogus, Elmas; Borcek, Pelin; Koca, Gokhan
    Introduction: To investigate the possible protective effect of lycopene on kidney after unilateral ureteral obstruction. Materials and methods: Twenty-one Wistar albino rats were divided into three groups in experimental study. In lycopene group, lycopene was administered two days before left ureteral ligation and was continued for 15 days. In control group, left ureteral ligation was applied with no medication. In sham group, midline incision was made to abdomen and then closed. Histopathological, biochemical and scintigraphic evaluations were made. Results: Microscopic score was 50.56 +/- 34.32 in lycopene, 97.22 +/- 39.14 in control and 28.33 +/- 12.58 in sham group. Microscopic score was lower in lycopene group than control group (p= 0.033). In biochemical analysis, mean value of myeloperoxidase (MPO), malondialdehyde (MDA) and nitrite levels were 0.05 +/- 0.03, 4.64 +/- 1.49 and 0.06 +/- 0.01 respectively in lycopene group, 0.10 +/- 0.03, 8.37 +/- 3.31 and 0.14 +/- 0.09 in control group and 0.04 +/- 0.01, 18.76 +/- 9.30 and 0.05 +/- 0.01 in sham group. MPO, MDA and nitrite levels were lower in lycopene group than in control group (p= 0.033, p= 0.007 and p < 0.001, respectively). Postoperative mean Technetium-99m dimercaptosuccinic acid (Tc-99m DMSA) uptake values of left kidneys were 6.26 +/- 5.17 in lycopene, 2.09 +/- 1.03 in control and 49.17 +/- 1.37 in sham group. There was no statistically significant difference between lycopene and control groups in respect of postoperative DMSA uptake values (p= 0.063). Conclusion: Lycopene is effective for the protection of kidney after ureteral obstruction as shown by microscopic parameters and decreased reactive oxygen species.
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    Scintigraphic, histopathologic, and biochemical evaluation of lycopene effects on renal ischemia reperfusion injury in rats
    (2017) Börcek, Pelin; Sadic, Murat; Atilgan, Hasan Ikbal; Aydin, Arif; Koca, Gokhan; Korkmaz, Meliha; Karakan, Tolga; Surer, Hatice
    Introduction/Objective Medical protection of kidneys against ischemia reperfusion injury is very important. Many agents have been used for the protection of ischemia reperfusion renal tissue injury. We aimed to evaluate the radioprotective effect of lycopene on kidneys in ischemia reperfusion injury with histopathological, biochemical, and scintigraphic parameters. Methods Twenty-one Wistar male albino rats were divided into the following three groups: lycopene, control, and sham group. In the lycopene group, lycopene was started three days before right renal ischemia reperfusion injury and continued for 15 days. In the control group, right renal ischemia reperfusion injury was applied with no medication. In the sham group, neither right renal ischemia reperfusion injury nor medication were applied. On the 15th day, all rats were sacrificed after Tc-99m-dimercaptosuccinic acid (DMSA) scintigraphies were taken. Histopathological, biochemical, and scintigraphic evaluations were made. Results The histopathological score was lower in the lycopene group. In biochemical analysis, myeloper-oxidase levels were lower in the lycopene group than in the control group, but not statistically significant. Malondialdehyde and nitrite levels were lower in the lycopene group than in the control group. The postoperative mean Tc-99m-DMSA uptake values were 44.82 +/- 1.84 in the lycopene group, 38.92 +/- 1.17 in the control group, and 50.21 +/- 1.35 in the sham group. DMSA uptake values were higher in the lycopene group than in the control group. Conclusion Lycopene seems to be an effective agent for protection of kidneys in ischemia reperfusion injury as demonstrated by the histopathological, biochemical, and scintigraphic parameters.