Browsing by Author "Atilgan, Alev Ok"

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Association between focal adhesion kinase and matrix metalloproteinase-9 expression in prostate adenocarcinoma and their influence on the progression of prostatic adenocarcinoma
(2020) Atilgan, Alev Ok; Ozdemir, B. Handan; Akcay, Eda Yilmaz; Tepeoglu, Merih; Borcek, Pelin; Dirim, Ayhan; 0000-0002-7528-3557; 0000-0001-8595-8880; 0000-0002-9894-8005; 0000-0001-6831-9585; 0000-0003-2898-485X; 32106037; X-8540-2019; AAK-3333-2021; AAK-5222-2021; AAK-1960-2021; AAJ-5689-2021
Focal adhesion kinase (FAK), a member of the non-receptor cytoplasmic tyrosine kinase family, is associated with the development and progression of cancer. Matrix metalloproteinase-9 (MMP-9) is directly involved in the degradation of the extracellular matrix, and basement membrane components promote cancer cell migration and invasion. There is a functional interaction among FAK, MMP-9 and vascular endothelial growth factor (VEGF), which leads to enhanced cancer angiogenesis, cancer cell invasion and progression of malignancy. FAK, MMP-9, VEGF and CD34-positive microvessel density (MVD) were examined in 100 patients with prostate adenocarcinoma using immunohistochemistry. The relationship among these proteins and their impact on angiogenesis and clinicopathological parameters were also evaluated. The FAK expression was found to be positively correlated with the Gleason score, WHO grade group, tumour stage, extracapsular extension and perineural invasion. The MMP-9 expression was positively correlated with the WHO grade group, tumour stage, extracapsular extension, positive surgical margin and lymphovascular and perineural invasion. The FAK expression was also positively correlated with MMP-9 expression and MVD. However, no correlation between FAK and VEGF expression was identified. The MMP-9 expression was positively correlated with FAK expression and MVD. Strong MMP-9 expression was associated with shorter disease-free survival. These results suggest that strong MMP-9 and FAK expressions play an essential role in the progression of prostate adenocarcinoma. Further investigations should be conducted to determine the importance of these proteins as therapeutic targets for patients with prostate adenocarcinomas.
COMPARISON OF RAPAMYCIN WITH CYCLOSPORIN, AND TACROLIMUS IN REGARDS TO THE DEVELOPMENT OF EPITHELIAL-TO-MESENCHYMAL TRANSITION (EMT) OF TUBULAR CELLS AND ENDOTHELIAL-TO-MESENCHYMAL TRANSITION (ENDOMT) OF PERITUBULAR CAPILLARIES (PTCS)
(2020) Ozdemir, B. Handan; Ozdemir, F. Nurhan; Atilgan, Alev Ok; Akcay, Eda; Polat, Aysegul Yucel; Akdur, Aydincan; Haberal, Mehmet A.
Comparison of Recipients with Early and Later Presentation Polyomavirus Nephropathy (PVN) with Regard to Histological Findings and Graft Survival: What is the Influence of CD4/CD8 Ration on the Presenting Time of PVN
(2018) Ozdemir, B. Handan; Ayva, Sebnem; Terzi, Aysen; Atilgan, Alev Ok; Akcay, Eda; Soy, Ebru H. Ayvazoglu; Acar, F. Nurhan Ozdemir; Haberal, Mehmet; 0000-0002-7528-3557; 0000-0002-2280-8778; 0000-0002-1225-1320; 0000-0001-8595-8880; 0000-0001-6831-9585; 0000-0002-0993-9917; 0000-0002-5682-0943; 0000-0002-3462-7632; X-8540-2019; AAK-1967-2021; F-7546-2013; AAK-3333-2021; AAK-1960-2021; AAC-5566-2019; AAK-1697-2021; AAJ-8097-2021
Divergent Role of Programmed Death -Ligand 1 (PD-L1) in Renal Allografts with Antibody-Mediated Rejection (AMR)
(2018) Ozdemir, B. Handan; Akcay, Eda; Atilgan, Alev Ok; Borcek, Pelin; Tepeoglu, Merih; Polat, Aysegul Yucel; Deniz, Melis; Haberal, Mehmet; https://orcid.org/0000-0002-7528-3557; https://orcid.org/0000-0001-6831-9585; https://orcid.org/0000-0001-8595-8880; https://orcid.org/0000-0002-9894-8005; https://orcid.org/0000-0002-3590-9375; AAJ-8097-2021; X-8540-2019; AAK-1960-2021; AAK-3333-2021; AAK-5222-2021; AAP-3975-2021; https://orcid.org/0000-0002-3462-7632
Divergent Role of Programmed Death-Ligand 1 in Renal Allografts with Antibody-Mediated Rejection
(2018) Ozdemir, B. Handan; Akcay, Eda Yilmaz; Atilgan, Alev Ok; Borcek, Pelin; Tepeoglu, Merih; Polat, Aysegul Yucel; Deniz, Isinsu Melis; Haberal, Mehmet; 0000-0002-7528-3557; 0000-0001-6831-9585; 0000-0001-8595-8880; 0000-0002-9894-8005; 0000-0002-3462-7632; X-8540-2019; AAK-1960-2021; AAK-3333-2021; AAK-5222-2021; AAJ-8097-2021
Effect of Adipose-Derived Stem Cells on Colonic Anastomosis in Immunosuppressed Rats with Everolimus: An Experimental Study
(2019) Karakaya, Emre; Akdur, Aydincan; Tezcaner, Tugan; Atilgan, Alev Ok; Uysal, Cagri; Ozer, Eda Ozturan; Yildirim, Sedat; Haberal, Mehmet A.
Effect of Adipose-Derived Stem Cells on Colonic Anastomosis in Rats Immunosuppressed With Everolimus: An Experimental Study
(2021) Karakaya, Emre; Akdur, Aydincan; Atilgan, Alev Ok; Uysal, Ahmet Cagri; Ozer, Huriye Eda Ozturan; Yildirim, Sedat; Haberal, Mehmet; 0000-0002-3462-7632; 0000-0002-8726-3369; 34269651; AAJ-8097-2021; AAA-3068-2021
Objectives: Immunosuppressed patients sometimes require colorectal surgery. We investigated whether adipose tissue-derived stem cells contributed to anastomosis healing in rats immunosuppressed with the mTOR inhibitor everolimus. Materials and Methods: Sixty male Sprague-Dawley rats were randomly divided into 4 groups of 14 each, with all groups undergoing descending colon anastomosis; the 4 remaining rats were used for stem cell retrieval. Group 1 (control) underwent surgery only, group 2 received stem cell injection, group 3 received everolimus only, and group 4 received everolimus plus stem cell injection. After treatment, each group was randomly divided into 2 equal subgroups according to the day of euthanasia (posttreatment day 4 or day 7). We measured anastomosis bursting pressure and tissue hydroxyproline level and performed histopathological evaluation. Results: At both posttreatment days 4 and 7, median weight loss in group 3 was higher than in group 1, group 3 had higher severity of intraabdominal adhesion than group 4, and group 2 had mean hydroxyproline level higher than the other groups. At posttreatment day 4, mean bursting pressure was significantly different in group 1 versus groups 2 and 4 (P = .002) and group 2 versus groups 3 and 4 (P < .001). No significant differences were shown in pathological analysis except for vascular proliferation on day 7 (P = .003). Conclusions: Injection of adipose tissue-derived stem cells in the anastomosis site prevented anastomosis leakage by contributing to healing. Injection of adipose tissue-derived stem cells in the anastomosis region, especially in the early period after solid-organ transplant in recipients and after gastrointestinal surgery in immunosuppressed patients, may help reduce mortality and morbidity.
Effect of Subcutaneous Topical Ozone Therapy on Second-Degree Burn Wounds in Rats: An Experimental Study
(2021) Karakaya, Emre; Akdur, Aydincan; Soy, Ebru Ayvazoglu; Araz, Coskun; Atilgan, Alev Ok; Ozer, Eda Ozturan; Sencelikel, Tugce; Haberal, Mehmet; 0000-0002-3462-7632; 0000-0002-0993-9917; 0000-0002-8726-3369; AAJ-8097-2021; AAC-5566-2019; AAA-3068-2021
Burns are one of the most severe traumas, causing coagulative destruction of the skin. The use of various products that accelerate wound healing in patients with burns may affect rates of patient survival and reduce complications. We studied the effects of subcutaneous ozone injection on second-degree burn wounds in an animal model. For this study, 72 Sprague-Dawley male rats were divided randomly into the following three groups: control group, silver sulfadiazine group, and ozone group; each group was then divided randomly into two subgroups (day 7 or day 14 examination and euthanized). Superficial partial-thickness burns were created on the lower back. In the control group, subcutaneous 0.9% serum saline was injected daily into the burn area. In the silver sulfadiazine group, burns were dressed daily with silver sulfadiazine. In the ozone group, subcutaneous ozone was injected daily into the burn area. We performed tissue hydroxyproline level measurements and histopathological evaluations. When groups were compared in terms of weight change, no significant difference was found between day 7 and day 14. With regard to tissue hydroxyproline levels, the ozone group had significantly higher levels on both days 7 and 14 (P < .001). In histopathological evaluations, we determined that wound healing in the ozone group was significantly higher than in the other groups. We found that subcutaneous ozone therapy was more effective than silver sulfadiazine in the healing process of second-degree burn wounds and could be safely used in the treatment of burn wounds.
Effects of Adipose-Induced Stem Cell Application on Everolimus Immunosuppressed Rats on Colon Anastomosis: An Experimental Study
(2018) Karakaya, Emre; Akdur, Aydincan; Atilgan, Alev Ok; Ozer, Huriye Eda Ozturan; Uysal, Cagri; Yildirim, Sedat; Haberal, Mehmet; https://orcid.org/0000-0002-4879-7974; https://orcid.org/0000-0002-8726-3369; https://orcid.org/0000-0001-8595-8880; https://orcid.org/0000-0002-5735-4315; https://orcid.org/0000-0002-3462-7632; AAD-5466-2021; AAA-3068-2021; AAK-3333-2021; AAF-4610-2019; AAJ-8097-2021
Effects of Adipose-Induced Stem Cell Application on Everolimus Immunosuppressed Rats on Colon Anastomosis: An Experimental Study
(2018) Karakaya, Emre; Akdur, Aydincan; Atilgan, Alev Ok; Ozer, Huriye Eda Ozturan; Uysal, Cagri; Yildirim, Sedat; Haberal, Mehmet; 0000-0002-4879-7974; 0000-0002-8726-3369; 0000-0001-8595-8880; 0000-0002-5735-4315; 0000-0002-3462-7632; AAD-5466-2021; AAA-3068-2021; AAK-3333-2021; AAF-4610-2019; AAJ-8097-2021
Endothelial To Myofibroblast Transition (Enmt) And Capillary Vegf Loss Enhances The Development Of Interstitial Fıbrosıs And Glomerulosclerosis
(2021) Ozdemir, Binnaz Handan; Akcay, Eda; Atilgan, Alev Ok; Haberal, Mehmet; 0000-0002-3462-7632; AAJ-8097-2021
Fine-Needle Aspiration Biopsy Findings of the Thyroid Gland in Renal Transplant Patients
(2023) Akcay, Eda Yilmaz; Tepeoglu, Merih; Atilgan, Alev Ok; Yagci, Sergen; Ozdemir, Binnaz Handan; Haberal, Mehmet; 37885286
Objectives: For patients with end-stage renal disease, thyroid diseases are common due to altered hormone excretion and transport, and for renal transplant recipients this is due to immunosuppressive drugs. We investigated the prevalence of thyroid disorders, including thyroid cancer, by fine-needle aspiration biopsy in kidney transplant candidates and recipients and estimated the outcomes. Materials and Methods: For 305 thyroid fine-needle aspiration biopsies performed from January 2000 to December 2020 in patients with end-stage renal disease, we recorded patient demographics, thyroid ultrasonography, and biopsy findings. Results: Of biopsy results from 305 patients, 272 (89.2%) were benign, 24 (7.9%) showed atypia of undetermined significance/follicular lesion of undetermined significance, 2 (0.7%) had suspicion for malignancy, and 7 (2.3%) were malignant. Thyroid surgery was performed for 13 patients with benign results, 6 with atypia of undetermined significance/follicular lesion of unde-termined significance, 2 with suspicion for malignancy, and 7 with malignancy. In 13 patients with benign cytology, the histopathology finding was also benign in lobectomy specimens. In 6 patients with atypia of undetermined significance/follicular lesion of undetermined significance, the final diagnosis was papillary thyroid carcinoma in 3 patients, adeno-matous hyperplasia in 2 patients, and Hurthle cell adenoma in 1 patient. For all 9 patients for whom fine-needle aspiration biopsy was suspicious for malignancy or malignant, histopathologic examination showed papillary thyroid carcinoma in total thyroidectomy materials. Among 12 papillary thyroid carcinoma patients, 4 underwent renal transplant after thyroidectomy, and survival for these 4 patients was 116.25 +/- 29.30 months after transplant without tumor recurrence or distant metastases. Conclusions: Thyroid diseases are more frequent in patients with end-stage renal disease or renal transplant versus the normal population and also affect morbidity and mortality at higher rates in these patients. Fine-needle aspiration biopsy is a useful diagnostic modality in evaluation and treatment of thyroid nodules in both kidney transplant candidates and recipients.
GLOMERULAR DEPOSITS OF VON WILLEBRAND FACTOR (VWF) AND LOSS OF ENDOTHELIAL CELL SURFACE ANTIGENS ON PERITUBULAR CAPILLARIES (PTCS) SHOWED A STRONG ASSOCIATION WITH WORSE GRAFT OUTCOME IN RECIPIENTS WITH ANTIBODY-MEDIATED REJECTION (ABMR)
(2020) Ozdemir, B. Handan; Ozdemir, F. Nurhan; Atilgan, Alev Ok; Akcay, Eda; Baskin, Eda; Haberal, Mehmet A.
High Cholesterol And Aging Negatively Influence Renal Capillary Density And Tubule Villin Expression By Decreasing Capillary Vegf And Nitric Oxide
(2021) Ozdemir, Binnaz Handan; Akcay, Eda; Atilgan, Alev Ok; Haberal, Mehmet; 0000-0002-3462-7632; AAJ-8097-2021
HYPERCHOLESTEROLEMIA (HC) INCREASES THE RISK OF ACUTE REJECTION (AR) AND LEADS TO CHRONIC REJECTION (CR) THROUGH DECREASING ENDOTHELIAL CELL SURFACE ANTIGENS AND INCREASING BOTH EPITHELIAL-TO-MESENCHYMAL TRANSITION (EMT) AND ENDOTHELIAL-TO-MESENCHYMAL TRANSITION (ENDOMT)
(2020) Ozdemir, B. Handan; Atilgan, Alev Ok; Ozdemir, Aydan Akyuz; Soy, Ebru H. Ayvazoglu; Haberal, Mehmet A.
THE IMPACT OF INVERTED RENAL CD4/CD8 RATIO IN THE FIRST 3-MONTH BIOPSIES POST-TRANSPLANT ON THE DEVELOPMENT OF TRANSPLANT GLOMERULOPATHY AND VASCULOPATHY IN RENAL ALLOGRAFTS
(2019) Ozdemir, Handan B.; Ozdemir, Nurhan F.; Atilgan, Alev Ok; Akcay, Eda; Kirna, Mahir; Haberal, Mehmet; AAH-9198-2019
The importance of age and CD4/CD8 ratio on the presenting time and the prognosis of the polyoma virus associated nephropathy (PVAN) after renal transplantation
(2019) Ozdemir, B. Handan; Ozdemir, F. Nurhan; Baskin, Esra; Atilgan, Alev Ok; Moray, Gokhan; Haberal, Mehmet; 0000-0003-4361-8508; B-5785-2018
In Vivo Study of The Role of Hyaluronic Acid, N-Acetyl Cysteine, and Deproteinized Calf Serum on Injury-Induced Cartilage Degeneration
(2023) Ozdemir, Mahmut; Birinci, Baris; Haberal, Bahtiyar; Atilgan, Alev Ok; Demirkale, Ismail; https://orcid.org/0000-0003-2674-9549; https://orcid.org/0000-0002-1668-6997; https://orcid.org/0000-0001-8595-8880; 36700278; HOH-2091-2023; W-9080-2019; AAK-3333-2021
Objectives: The aim of this study was to compare the effects of hyaluronic acid ( HA), N-acetyl cysteine ( NAC), and deproteinized calf serum on cartilage healing after the creation of traumatic cartilage injury in a rat model. Materials and methods: A total of 48 rats, each weighing an average of 350 g, were randomly separated into four groups of 12. An osteochondral defect was created, 2-mm-wide and 3-mm deep in each rat. Injections were made to the knees of the rats as saline solution in Group 1, deproteinized calf serum in Group 2, NAC in Group 3, and HA in Group 4. At the end of 12 weeks, all rats were sacrificed and tissues were evaluated histologically. Results: The HA group had a better cell morphology, tissue morphology, surface architecture, and vascularity than the other groups (p < 0.001). Matrix staining, chondrocyte clustering, and the assessment scores of the mid, deep, superficial zones, and overall were higher in the HA group than in the other groups (p < 0.001). The NAC showed a better tissue morphology, cell morphology, and vascularity than the control group (p= 0.003, p < 0.001, and p < 0.001, respectively). Conclusion: Hyaluronic acid was the most effective agent in cartilage healing compared to NAC and deproteinized calf serum. In addition, the NAC was more effective compared to the control group.
The Influence of Polyomavirus Infected Glomerular Cells on the Development of Glomerulopathy and the Graft Survival
(2018) Ozdemir, B. Handan; Terzi, Aysen; Ayva, Sebnem; Akcay, Eda; Atilgan, Alev Ok; Soy, Ebru H. Ayvazoglu; Acar, F. Nurhan Ozdemir; Moray, Gokhan; Haberal, Mehmet; 0000-0002-7528-3557; 0000-0002-1225-1320; 0000-0002-2280-8778; 0000-0001-6831-9585; 0000-0001-8595-8880; 0000-0002-0993-9917; 0000-0002-5682-0943; 0000-0003-2498-7287; 0000-0002-3462-7632; X-8540-2019; F-7546-2013; AAK-1967-2021; AAK-1960-2021; AAK-3333-2021; AAC-5566-2019; AAK-1697-2021; AAE-1041-2021; AAJ-8097-2021
Intraosseous angiolipoma of the frontal bone with a unique location: A clinical and pathological case illustration and review of the literature
(2014) Atilgan, Alev Ok; Terzi, Aysen; Agildere, Muhtesem; Caner, Hakan; Ozdemir, B. Handan
Here, we report a case of a 16-year-old female patient was referred with scalp swelling and headache. Her neurological examination was normal and imaging of the skull revealed a well-defined lytic lesion measuring 15 mm x 6 mm to the right of the frontal bone. She was operated on with a prediagnosis of Langerhans cell histiocytosis. A wide excision with negative margins was made and the defect was reconstructed with a titanium plate. Subsequently, the lesion was histopathologically diagnosed as an angiolipoma of the frontal bone. The postoperative period was uneventful and she remained well during 1-year follow-up with no evidence of recurrence. Angiolipomas are rare benign lipomatous lesions located mostly in subcutaneous tissue of the forearm or trunk and frequently occur before puberty or in young adults. They are not common in bones. To the best of our knowledge, this is the first angiolipoma of the frontal bone reported.