Browsing by Author "Akcay, Ali"

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    Böbrek nakli yapılan hastalarda hiperürisemi ve gut hastalığı sıklığı
    (Tıp Araştırmaları Dergisi 2006 4 (2):7-10, 2006) Kanbay, Mehmet; Usluoğulları, Alper; Huddam, Bulent; Çolak, Turan; Akcay, Ali; Kart-Köseoğlu, Hamide; Yücel, Eftal; Haberal, Mehmet
    Amaç: Böbrek nakli sonrası hiperürisemi sık görülmesine rağmen gut hastalığı nadirdir. Ancak bu konu ile ilgili böbrek nakli yapılmış Türk hasta grubunda yayınlanmış makale mevcut değildir. Bu çalışmanın amacı böbrek nakli yapılmış hastalarda hiperürisemi ve gut hastalığı görülme sıklığını belir-lemektir. Gereç ve Yöntem: Başkent Üniversitesi Hastanesi Transplantasyon Ünitesinde 2000 ve 2002 yılları arasında böbrek nakli yapılmış 155 hasta (E/K, 119/36; ortalama yaş 34,7±9,7 yıl) retrospektif olarak araştırıldı. Çalışmaya en az 2 yıl süresince normal böbrek fonksiyonlarına sahip olan hastalar dahil edildi. Hastaların nakil olduktan sonra rutin poliklinik kontrollerindeki laboratuvar değerleri kayıt edildi. Ayrıca hastaların demografik özellikleri ve kullandığı ilaçlar kayıt edildi. Serum ürik asid seviyesi kadınlarda 6 mg/dl erkeklerde 7 mg/dl üzerinde ise hiperürisemi olarak kabul edildi. Klinik olarak gut hastalığı hiperürisemi ile birlikte gut artritinin ve tofüsün olmasıyla tanımlandı. Bulgular: Hiperürisemi 95 hastada, gut hastalığı ise 13 hastada saptandı. Serum ürik asid seviyelerinin hastaların yaşından, cinsiyetinden, donörün canlı veya kadavra olmasından ve aldıkları ilaç rejimin-den bağımsız olduğu saptandı. Sonuç: Böbrek transplantasyonu yapılan hastalarda hiperürisemi yaygın olarak görülmesine rağmen gut hastalığı bu hasta grubunda nadirdir. Aim: Although the prevalence of hyperuricemia is high after renal transplantation, investigation has shown that gout occurs rarely in these patients. The present study was designed to investigate the preva-lence of hyperuricemia and gout in renal transplant patients. Materials and methods: The records of 155 patients (M/F, 119/36, mean age 34.7 9.7 years) who under-went renal transplantation in between 2000 and 2002 were retrospectively reevaluated. Patients with at least 2 years stable graft survival duration were included. For each individual, mean value of serum uric acid levels that were repeated in each routine visits approximately every 6 months in transplanta-tion outpatient clinic were used. Patient demograph-ics, immunosuppressive drug regimens and other medications were also recorded. Hyperuricemia was defined as serum uric acid level of >6 mg/dl in females and 7 mg/dl in males. Clinical gout was defined as hyperuricemia with gouty arthritis and tophi. Results: Hyperuricemia and gout were seen in 95 patients, and 13 patients, respectively. Mean serum uric acid levels were found to be independent from patients' age, sex, donor type, and immunosuppres-sive drug regimen. Conclusion: Our study confirmed that although hyperuricemia is a common complication in renal transplant recipients, gout is not seen often in these populations.
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    Role of autophagy and evaluation the effects of microRNAs 214, 132, 34c and prorenin receptor in a rat model of focal segmental glomerulosclerosis
    (2021) Yildirim, Derya; Bender, Onur; Karagoz, Zehra Firat; Helvacioglu, Fatma; Bilgic, Mukadder Ayse; Akcay, Ali; Ruzgaresen, Nuket Bavbek; 0000-0002-6026-0045; 34087284
    Aims: Focal segmental glomerulosclerosis (FSGS) is the common cause of chronic renal disease worldwide. Although there are many etiologic factors which have common theme of podocyte injury conclusive etiology is not clearly understood. In this study, we aimed to explore the role of autophagy in the pathogenesis of podocyte injury, which is the key point in disease progression, and the roles of intrarenal microRNAs and the prorenin receptor (PRR) in the 5/6 nephrectomy and adriamycin nephropathy models of FSGS. Main methods: For experimental FSGS model, 5/6 nephrectomy and adriamycin nephropathy models were created and characterized in adult Sprague Dawley rats. Microarray analysis was performed on FSGS and control groups that was confirmed by q-RT-PCR. Beclin1, LC3B, PRR, ATG7 and ATG5 expression were evaluated by western blotting and immunohistochemistry. Also, Beclin1 and PRR expression were measured by ELISA. Glomerular podocyte isolation was performed and autophagic activity was evaluated in podocytes before and after transfection with miRNA mimic and antagonists. Key findings: Glomerular expression of Beclin1, LC3B, PRR, ATG7 and ATG5 were significantly lower in the 5/6 nephrectomy than adriamycin nephropathy group and in both groups lower when compared to control groups. Western blot results were consistent with immunohistochemical data. Electron microscopy revealed signs of impaired autophagy in FSGS. Autophagic activity decreased significantly after miR-214, miR-132 and miR-34c mimics and increased after transfection with antagonists. Significance: These results showed that the role of autophagic activity and decreased expression of PRR in FSGS pathogenesis and miR-34c, miR-132 and miR-214 could be a potential treatment strategy by regulating autophagy.