Scopus İndeksli Yayınlar Koleksiyonu
Permanent URI for this collectionhttps://hdl.handle.net/11727/4809
Browse
2 results
Search Results
Item Comparison of Antimicrobial Susceptibilities of Escherichia coli Isolated From Urinary Cultures of Different Patient Groups: A University Hospital Experience(2020) Mirza, Hasan Cenk; Sancak, Banu; F-1232-2015Objective: Our objective was to investigate the antimicrobial susceptibilities of Escherichia coli isolated from urinary cultures in Central Laboratory of Hacettepe University Faculty of Medicine Hospital and to examine the differences between antimicrobial susceptibilities of E. coli isolated from different patient groups. Methods: E. coli isolated from urinary cultures between January 1, 2017 and April 30, 2018 were included in our study. Automated systems, i.e. VITEK (R) 2 Compact (bioMerieux, Marcy l'Etoile, France) and BD Phoenix (Becton Dickinson, Sparks, MD, USA) and disk diffusion test were used for the determination of antimicrobial susceptibilities. The patients from whom the bacteria were isolated were divided into groups according to age (<18 years, 18-64 years, and >64 years), gender and patient care (outpatients/inpatients). Results: The highest susceptibility rates were observed for carbapenems (>99%), fosfomycin (98.5%), nitrofurantoin (98.3%) and amikacin (94.2%), whereas the highest resistance rates were observed for ampicillin (61.3%) and amoxicillin-clavulanate (37.5-45.7%). Antimicrobial resistance rates of isolates from patients aged 65 years and over were higher than those of patients in other age groups, with the exception of piperacillintazobactam, amikacin and ertapenem. The resistance rates of isolates belonging to male patients were higher than those belonging to female patients for all antimicrobials. Also, the resistance rates of isolates belonging to inpatients were higher than those belonging to outpatients for all antimicrobials. When the rates of extended-spectrum beta-lactamase (ESBL)-producing E. coli from different age groups were compared, the highest rate (34.2%) was observed among the isolates from patients aged 65 years and over. The rates of ESBL-producing E. coli from males (33.9%) and inpatients (36.3%) were higher than those from females (23.8%) and outpatients (23.3%), respectively. Conclusions: Antimicrobial susceptibilities of E. coli isolates may vary among different patient groups. Demographic features of patients may guide for selecting the antimicrobials for empiric treatment of urinary tract infections.Item Bacterial detection using bacteriophages and gold nanorods by following time-dependent changes in Raman spectral signals(2018) Moghtader, F.; Tomak, A.; Zareie, H.M.; Piskin, E.; 29583029This study attemps to develop bacterial detection strategies using bacteriophages and gold nanorods (GNRs) by Raman spectral analysis. Escherichia coli was selected as the target and its specific phage was used as the bioprobe. Target bacteria and phages were propagated/purified by traditional techniques. GNRs were synthesized by using hexadecyltrimethyl ammonium bromide (CTAB) as stabilizer. A two-step detection strategy was applied: Firstly, the target bacteria were interacted with GNRs in suspensions, and then they were dropped onto silica substrates for detection. It was possible to obtain clear surface-enchanced Raman spectroscopy (SERS) peaks of the target bacteria, even without using phages. In the second step, the phage nanoemulsions were droped onto the bacterial–GNRs complexes on those surfaces and time-dependent changes in the Raman spectra were monitored at different time intervals upto 40 min. These results demonstrated that how one can apply phages with plasmonic nanoparticles for detection of pathogenic bacteria very effectively in a quite simple test. © 2018, © 2018 Informa UK Limited, trading as Taylor & Francis Group.