Gulsahi, KamranTirali, R. EbruCehreli, S. BurcakKarahan, Zeynep CerenUzunoglu, EmelSabuncuoglu, Bizden2024-03-142024-03-1420141618-1247http://hdl.handle.net/11727/11820The aim of this study was to evaluate the effectiveness of 2.5 % NaOCl at different temperature and time intervals on Enterococcus faecalis and Candida albicans-infected human roots. A total of 112 root cylinders prepared from extracted single-rooted humans were infected by E. faecalis (Group A, n = 56) or C. albicans (Group B, n = 56); 3 root cylinders served as negative controls. Both groups were further divided into 6 subgroups according to three contact times (30 s, 1 min, 5 min) with NaOCl at two different temperatures (25 or 37 A degrees C). Microorganism growth was controlled at the 24th and 48th hours. Statistical analysis was performed using the Chi-square test. While NaOCl at 25 A degrees C for 5 min was the most effective irrigation regimen to eliminate E. faecalis (p < 0.001), NaOCl at 37 A degrees C for 5 min exhibited significantly superior antifungal properties (p < 0.05). At the same contact times, difference in the temperature of NaOCl did not affect the growth of either E. faecalis or C. albicans. As a result, the irrigation time of NaOCl was more effective than the temperature to eliminate E. faecalis, while pre-heating of NaOCl to 37 A degrees C increased its effectiveness on C. albicans at 5 min contact time.enginfo:eu-repo/semantics/closedAccessSodium hypochloritePreheatingC. albicansE. faecalisarticle102136410003307347000052-s2.0-84892863775