Show simple item record

dc.contributor.authorKayar, Nezahat Arzu
dc.contributor.authorOduncuoglu, Bahar Fusun
dc.contributor.authorHalilolgu, Seyfullah
dc.contributor.authorSerpek, Behic
dc.contributor.authorAtaoglu, Tamer
dc.contributor.authorAlptekin, Nilgun Ozlem
dc.date.accessioned2020-07-28T12:47:45Z
dc.date.available2020-07-28T12:47:45Z
dc.date.issued2019
dc.identifier.issn0001-6357en_US
dc.identifier.urihttp://hdl.handle.net/11727/4799
dc.description.abstractObjectives: The mechanisms underlying the formation and composition of gingival crevicular fluid (GCF) and its flow into and from periodontal pockets are not understood very well. The aim of this study was to evaluate the length of sampling time and sequential sampling of GCF neutrophil elastase (NE) enzyme levels by using intracrevicular and orifice methods. Material and methods: Twenty adults (mean age of 41.8 years, ranged 31-60 years, 18 males and 2 females) with chronic periodontitis were enrolled and all completed the 3-d study. GCF was collected by both intracrevicular and intrasulcular methods, 720 samples of GCF were collected. In first, second and third day, the length of sampling time in seconds (s) and order were '5- 10-30-s'; '10- 30- 5-s' and '30- 5- 10-s,' respectively. GCF elastase levels were determined by hydrolysis of neutrophil specific substrate N-methoxysuccinyl-Ala-Ala-Pro-Val-p-nitroanilide. Results: NE activity (mu U) and NE activity/volume (mu U/mu l) were significantly different for order of sampling (p < .05), but not for the length of sampling time (p>.05). Conclusions: Within the limits of this study, the choice of sampling technique in GCF-profile studies seems to be a critical decision as it has the potential to affect the GCF volume and NE activity.en_US
dc.language.isoengen_US
dc.relation.isversionof10.1080/00016357.2019.1703141en_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectGingival crevicular fluiden_US
dc.subjectsampling methodsen_US
dc.subjectsequential samplingen_US
dc.subjectsamplingen_US
dc.titleMethodological evaluation of gingival crevicular fluid volume and neutrophil elastase levels: sequential sampling, length of sampling time and two different sampling methodsen_US
dc.typearticleen_US
dc.relation.journalACTA ODONTOLOGICA SCANDINAVICAen_US
dc.identifier.volume78en_US
dc.identifier.issue4en_US
dc.identifier.startpage290en_US
dc.identifier.endpage296en_US
dc.identifier.wos000503362900001en_US
dc.identifier.scopus2-s2.0-85083523804en_US
dc.contributor.pubmedID31852321en_US
dc.contributor.orcID0000-0003-0647-9481en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergien_US
dc.contributor.researcherIDAAH-5405-2020en_US
dc.contributor.researcherIDAAQ-4792-2020en_US


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record