dc.contributor.author | Akbulut, Sami | |
dc.contributor.author | Sevmis, Sinasi | |
dc.contributor.author | Karayakali, Hamdi | |
dc.contributor.author | Bayraktar, Nilufer | |
dc.contributor.author | Unlukaplan, Muge | |
dc.contributor.author | Oksuz, Ergun | |
dc.contributor.author | Dagdeviren, Atilla | |
dc.date.accessioned | 2019-12-18T14:10:17Z | |
dc.date.available | 2019-12-18T14:10:17Z | |
dc.date.issued | 2014 | |
dc.identifier.issn | 1007-9327 | |
dc.identifier.issn | 1300-7777 | |
dc.identifier.uri | https://www.wjgnet.com/1007-9327/full/v20/i34/12292.htm | |
dc.identifier.uri | http://hdl.handle.net/11727/4477 | |
dc.description.abstract | AIM: To investigate whether amifostine contributes to the antioxidant and cytoprotective effects of histidine-tryptophan-ketoglutarate (HTK) and University of Wisconsin (UW) preservation solutions.
METHODS: Forty-eight Sprague Dawley male rats were equally divided into six groups: (1) ringer Lactate (RL) group; (2) RL + amifostine (RL + A) group; (3) HTK group; (4) HTK + A group; (5) UW group; and (6) UW + A group. Rats in the RL + A, HTK + A and UW + A groups were administered amifostine intraperitoneally at a dose of 200 mg/kg prior to laparotomy. The RL group was perfused with RL into the portal vein. The RL + A group were perfused with RL into the portal vein after amifostine administration. The HTK group received an HTK perfusion while the HTK + A group received an HTK perfusion after administration of amifostine. The UW group received a perfusion of UW, while the UW + A group received a UW perfusion after amifostine administration. Liver biopsy was performed to investigate histopathological, immunochemical [transferase mediated dUTP nick end labeling (TUNEL), inducible nitric oxide syntetase (iNOS)] and ultrastructural alterations. Biochemical alterations were determined by examining levels of alanine aminotransferase, alkaline phosphatase and nitric oxide in the perfusion fluid.
RESULTS: Pathological sinusoidal dilatation and centrilobular hydropic alteration were significantly lower in the groups that received amifostine prior to preservation solution perfusion. Although the best results were obtained in the UW + A group, we did not observe a statistically significant difference between the UW + A and HTK + A groups. iNOS grades were significantly lower in the amifostine groups 12 h after treatment. When the amifostine groups were compared against each other, the iNOS grades obtained from the UW + A and HTK + A groups were similar while the RL + A group had a much poorer score. TUNEL assays demonstrated a lower apoptosis ratio in the amifostine groups than in the non-amifostine groups 12 h after treatment. No statistically significant difference was observed between the UW + A and HTK + A groups for apoptosis. Cellular ultrastructure was best preserved in the UW + A and HTK + A groups.
CONCLUSION: Here, we show that preoperative administration of a single dose of amifostine is sufficient to minimize the preservation damage in hepatic cells. (C) 2014 Baishideng Publishing Group Inc. All rights reserved. | en_US |
dc.language.iso | eng | en_US |
dc.relation.isversionof | 10.3748/wjg.v20.i34.12292 | en_US |
dc.rights | info:eu-repo/semantics/openAccess | en_US |
dc.subject | Liver | en_US |
dc.subject | Transplantation | en_US |
dc.subject | Preservation solutions | en_US |
dc.subject | Histidine-tryptophan-ketoglutarate | en_US |
dc.subject | University of Wisconsin | en_US |
dc.subject | Antioxidant | en_US |
dc.subject | Amifostine | en_US |
dc.title | Amifostine enhances the antioxidant and hepatoprotective effects of UW and HTK preservation solutions | en_US |
dc.type | article | en_US |
dc.relation.journal | WORLD JOURNAL OF GASTROENTEROLOGY | en_US |
dc.identifier.volume | 20 | en_US |
dc.identifier.issue | 34 | en_US |
dc.identifier.startpage | 12292 | en_US |
dc.identifier.endpage | 12300 | en_US |
dc.identifier.wos | 000341719100038 | en_US |
dc.identifier.wos | 000341678000012 | |
dc.identifier.scopus | 2-s2.0-84909609683 | en_US |
dc.contributor.pubmedID | 25232264 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi | en_US |