HUMAN PAPILLOMA VIRUS (HPV) PREVALENCE AND GENOTYPE DISTRIBUTION

Abstract

Assessment of Human papillomavirus (HPV) prevalence and genotype distribution is important for monitoring the impact of prophylactic HPV vaccination. This study aimed to demonstrate the HPV prevalence and type distribution in women from the Baskent University Adana Dr. Turgut Noyan Practice and Research Center. Cervical specimens from 268 women aged 22-68 years were collected between April 2014 and November 2015. Histopathological examinations were performed for 146 women. HPV DNA was amplified by PCR and HPV and genotyping was undertaken using the Roche (R) linear array detection kit. In total. 124 out of 268eligible samples (46.31%) tested positive for HPV. with the majority of these [84/124 (67.7%1] having high-risk (HR) HPV infection: 20.9% were positive for HPV16 (n=261, and 4% for HPV18 (n=5) HPV type-specific prevalence was 63.9% 53.39 and 80% among cervical intraepithelial neoplasias (CIN) Grades respectively. The coverage of other HR-HPV genotypes apart from 16. included HPV31. 45. 51, 53, and 56 in high-grade cervical lesions. In conclusion, HPV-16 was identified as the main HPV genotype associated with cervical disease in our hospital. The study reports the identification of high-and low-risk HPV genotypes as well as the prevalence of multiple HPV infections.